Keles Hikmet, Yalcin Alper, Aydin Hasan
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Department of Histology, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey.
Arch Med Sci. 2019 Jul 22;18(6):1659-1665. doi: 10.5114/aoms.2019.86776. eCollection 2022.
The degenerative effects of imidacloprid via oxidative stress are known. Irisin is a recently discovered peptide with energy regulator and antioxidant effects. In addition, the antioxidant potential of Vitamin D has been reported in previous studies. The current study was performed to investigate the effect of Vitamin D on testis morphology and irisin immunoreactivity in imidacloprid-treated rats.
Thirty-two Wistar albino male rats were divided into groups: control ( = 6), corn oil ( = 6), Vitamin D ( = 6), imidacloprid ( = 7) and imidacloprid + Vitamin D ( = 7). Testis tissues were used to evaluate the histopathological, biochemical and immunohistochemical changes. Oxidative state in testis tissue was determined with total antioxidant and oxidant status markers, total antioxidant status (TAS) and total oxidant status (TOS) respectively.
In microscopic examination, degenerative changes in the seminiferous tubule epithelium, interstitial edema and increased irisin immunoreactivity were observed in animals given imidacloprid. Also increased TOS and decreased TAS levels were measured in these animals. It was observed that Vitamin D improved the testicular damage histopathologically when compared to the imidacloprid group. However, increase in TAS levels and decrease in both TOS levels and irisin immunoreactivity were found insignificant in animals given Vitamin D.
In the present study it was observed that Vitamin D ameliorated testis injury caused by imidacloprid. Furthermore, imidacloprid was found to increase the immunoreactivity of irisin. In the light of our findings, we conclude that the use of Vitamin D could be beneficial against testicular damage caused by imidacloprid.
吡虫啉通过氧化应激产生的退化作用是已知的。鸢尾素是最近发现的一种具有能量调节和抗氧化作用的肽。此外，先前的研究报道了维生素D的抗氧化潜力。本研究旨在探讨维生素D对吡虫啉处理大鼠睾丸形态和鸢尾素免疫反应性的影响。
将32只Wistar白化雄性大鼠分为几组：对照组（n = 6）、玉米油组（n = 6）、维生素D组（n = 6）、吡虫啉组（n = 7）和吡虫啉 + 维生素D组（n = 7）。使用睾丸组织评估组织病理学、生化和免疫组化变化。分别用总抗氧化和氧化剂状态标志物总抗氧化状态（TAS）和总氧化剂状态（TOS）测定睾丸组织中的氧化状态。
在显微镜检查中，给予吡虫啉的动物出现生精小管上皮的退化性变化、间质水肿和鸢尾素免疫反应性增加。这些动物的TOS水平也升高，TAS水平降低。与吡虫啉组相比，观察到维生素D在组织病理学上改善了睾丸损伤。然而，给予维生素D的动物中TAS水平的升高以及TOS水平和鸢尾素免疫反应性的降低均无显著意义。
在本研究中，观察到维生素D改善了吡虫啉引起的睾丸损伤。此外，发现吡虫啉增加了鸢尾素的免疫反应性。根据我们的研究结果，我们得出结论，使用维生素D可能对吡虫啉引起的睾丸损伤有益。
