白细胞介素-8信使核糖核酸的合成及蛋白质分泌被呼吸道合胞病毒持续感染的细胞持续上调。

Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells.

作者信息

Tirado Rocio, Ortega Arturo, Sarmiento Rosa Elena, Gómez Beatríz

机构信息

Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Cd. Universitaria, Mexico D.F. 04510, Mexico.

出版信息

Cell Immunol. 2005 Jan;233(1):61-71. doi: 10.1016/j.cellimm.2005.04.003.

DOI:10.1016/j.cellimm.2005.04.003

PMID:15936741

Abstract

The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.

摘要

本研究的目的是调查呼吸道合胞病毒持续感染是否会调节人肺上皮细胞系（A549）中白细胞介素8（IL-8）的mRNA合成和蛋白质分泌。因此，我们在这些细胞中建立了呼吸道合胞病毒持续感染（A549per），并通过逆转录聚合酶链反应（RT-PCR）测定白细胞介素-8 mRNA的水平，通过酶联免疫吸附测定（ELISA）测定蛋白质水平。在传代过程中，A549per细胞以及与从A549per传代获得的上清液（cA549per）孵育的A549细胞中，白细胞介素-8 mRNA合成和蛋白质分泌持续上调。这些结果表明，白细胞介素-8的增强是由细胞中呼吸道合胞病毒基因组的存在或由呼吸道合胞病毒诱导的可溶性介质刺激的，这反过来又增加了白细胞介素-8 mRNA的合成和蛋白质分泌。可溶性呼吸道合胞病毒F和G蛋白被鉴定为介质。此外，与可溶性介质孵育1分钟后观察到白细胞介素-8增强，因此表明白细胞介素-8的上调是由受体-配体相互作用触发的。