Uhlenhaut Christine, Kracht Maren
Center for Biological Safety, Robert Koch-Institute, Nordufer 20, 13353 Berlin, Germany.
J Virol Methods. 2005 Sep;128(1-2):189-91. doi: 10.1016/j.jviromet.2005.05.002.
RNA stabilization buffers are valuable additives which are used widely. Although their effect on RNA protection has been well assessed, the impact on viral infectivity was unsettled. The potential stabilizing or inactivating effects of RNAlater (Qiagen) on the infectivity of different relevant and model viruses including HIV were assessed. Our results show that RNAlater not only protects RNA as published by others but also stabilizes virus infectivity of enveloped and non-enveloped model viruses for a considerable period of time on a relatively high level (storage at room temperature for 50 days yielded infectious titers > 100 TCID50).
RNA稳定缓冲液是广泛使用的重要添加剂。尽管它们对RNA保护的作用已得到充分评估，但对病毒感染性的影响仍未确定。我们评估了RNA later（Qiagen公司）对包括HIV在内的不同相关病毒和模型病毒感染性的潜在稳定或灭活作用。我们的结果表明，RNA later不仅如其他人所报道的那样能保护RNA，而且还能在相当长的一段时间内将包膜和非包膜模型病毒的感染性稳定在相对较高的水平（在室温下储存50天，感染滴度>100 TCID50）。
