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在疫情环境中用于病毒组测序的保存方法

Use of Preservation for Virome Sequencing in Outbreak Settings.

作者信息

Kohl Claudia, Wegener Merle, Nitsche Andreas, Kurth Andreas

机构信息

Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.

出版信息

Front Microbiol. 2017 Sep 26;8:1888. doi: 10.3389/fmicb.2017.01888. eCollection 2017.

DOI:10.3389/fmicb.2017.01888
PMID:29018436
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5623189/
Abstract

Outbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of -treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in buffer yield similar results to those stored snap-frozen.

摘要

传染病疫情可能在动物和人类群体中发生；这凸显了采用合适的“同一健康”方法的必要性。在疫情爆发期间，直接鉴定病原体对于采取应对措施至关重要。最近发布的一项用于临床样本宏基因组病毒检测的方案（TUViD-VM）是针对难以获取的速冻组织开发的。在此，我们描述了在常温下使用经 - 处理的组织进行病毒组测序。这项研究表明，保存在 缓冲液中的样本产生的结果与速冻保存的样本相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb3/5623189/10e609e11be7/fmicb-08-01888-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb3/5623189/dbce5feb94a5/fmicb-08-01888-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb3/5623189/10e609e11be7/fmicb-08-01888-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb3/5623189/dbce5feb94a5/fmicb-08-01888-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eb3/5623189/10e609e11be7/fmicb-08-01888-g002.jpg

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本文引用的文献

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2
Optimization of preservation and storage time of sponge tissues to obtain quality mRNA for next-generation sequencing.优化海绵组织的保存和储存时间，以获得用于下一代测序的高质量 mRNA。
Mol Ecol Resour. 2012 Mar;12(2):312-22. doi: 10.1111/j.1755-0998.2011.03097.x. Epub 2011 Dec 4.
3
Possible biohazard risk from infectious tissue and culture cells preserved with RNAlater.
采用系统微生物学方法进行革命性的病毒组学研究。
Exp Biol Med (Maywood). 2022 Jul;247(13):1135-1147. doi: 10.1177/15353702221102895. Epub 2022 Jun 20.
4
The virome of German bats: comparing virus discovery approaches.德国蝙蝠的病毒组：比较病毒发现方法。
Sci Rep. 2021 Apr 1;11(1):7430. doi: 10.1038/s41598-021-86435-4.
5
Potential Applications of Human Viral Metagenomics and Reference Materials: Considerations for Current and Future Viruses.人类病毒宏基因组学及参考材料的潜在应用：针对当前和未来病毒的考量
Appl Environ Microbiol. 2020 Oct 28;86(22). doi: 10.1128/AEM.01794-20.
6
Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation.全面的蛋白质组和磷酸化蛋白质组分析表明，RNA Later对蛋白质丰度和磷酸化的影响可忽略不计。
Clin Proteomics. 2019 Apr 25;16:18. doi: 10.1186/s12014-019-9239-z. eCollection 2019.
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