Sathish Narayanan, Abraham Priya, Peedicayil Abraham, Sridharan Gopalan, Chandy George
Department of Clinical Virology, Christian Medical College, Vellore 632004, India.
Cancer Lett. 2005 Nov 8;229(1):93-9. doi: 10.1016/j.canlet.2005.04.026.
In a cross-sectional study performed between June 2001 and November 2003, the HPV 16 E6 gene of 50 women with cervical neoplasia and 20 cytologically normal women ('controls') was sequenced following amplification by PCR. The 350T to G variant was seen in 35 (70%) of 50 patients' isolates while it was seen in only 3 (15%) of the 20 isolates from the 'controls'. The higher occurrence of the 350G variant among the patients was statistically significant (P<0.01). Isolates from patients were grouped into the European (E), Asian-American (AA) and North-American (NA-1) phylogenetic clusters with 46 (92%) belonging to the E cluster. All the 20 isolates from 'controls' belonged to the E cluster. This study suggests an association of the HPV 16 350G variant with a higher risk of cervical neoplasia and a predominance of E lineage strains among Indian HPV 16 isolates.
在2001年6月至2003年11月期间进行的一项横断面研究中，对50例宫颈肿瘤患者和20例细胞学正常女性（“对照”）的HPV 16 E6基因进行了PCR扩增后的测序。50例患者分离株中有35例（70%）出现了350T到G的变异，而在20例“对照”分离株中仅有3例（15%）出现该变异。患者中350G变异的较高发生率具有统计学意义（P<0.01）。患者的分离株被分为欧洲（E）、亚裔美国人（AA）和北美（NA-1）系统发育簇，其中46例（92%）属于E簇。“对照”的所有20例分离株均属于E簇。本研究表明，HPV 16 350G变异与宫颈肿瘤的较高风险相关，且在印度HPV 16分离株中E谱系菌株占优势。
