Pittman Phillip R, Leitman Susan F, Oro Julio G Barrera, Norris Sarah L, Marano Nina M, Ranadive Manmohan V, Sink Bonnie S, McKee Kelly T
U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, MD 21702-500, USA.
Clin Diagn Lab Immunol. 2005 Jun;12(6):713-21. doi: 10.1128/CDLI.12.6.713-721.2005.
Recipients of licensed anthrax vaccine (AVA; Biothrax) could serve as a source of hyperimmune plasma and immunoglobulin for therapy and prophylaxis. We measured serum antibodies during serial weekly to biweekly plasmapheresis in 38 individuals previously vaccinated with 4 to 27 doses of AVA. Immunoglobulin G (IgG) to protective antigen (PA) and toxin neutralization assay (TNA) antibody levels were highly correlated (r = 0.86930 and P < 0.0001 for anti-PA concentration versus TNA concentration). Significant decreases in antibody titer and concentration were observed over time when compared for the number of days from the last AVA injection (P < 0.0001 for both anti-PA and TNA concentration) and for the number of days from the first plasmapheresis (P = 0.0007 for anti-PA concentration and P = 0.0025 for TNA concentration). The rate of the decrease in total IgG concentration (half-life [t(1/2)] = 198.90 days after first plasmapheresis) was significantly less than the decrease in anti-PA IgG (t(1/2) = 63.53 days) (P < 0.0001), indicating that the reduction in anti-PA IgG was more likely due to natural decay than plasmapheresis. The time since the last injection and the time after initial plasmapheresis are important elements in considering an optimal schedule for collecting anthrax hyperimmune plasma. Good correlation between IgG to PA and TNA antibodies suggests that the anti-PA enzyme-linked immunosorbent assay can be used as a high-throughput screen for functional immune reactivity in donor plasma units.
已获许可的炭疽疫苗（AVA；BioThrax）接种者可作为治疗和预防用超免疫血浆及免疫球蛋白的来源。我们在38名先前接种过4至27剂AVA的个体中，每周至每两周进行一次连续血浆置换期间测量血清抗体。针对保护性抗原（PA）的免疫球蛋白G（IgG）和毒素中和试验（TNA）抗体水平高度相关（抗PA浓度与TNA浓度的r = 0.86930，P < 0.0001）。与自上次AVA注射后的天数（抗PA和TNA浓度均P < 0.0001）以及自首次血浆置换后的天数（抗PA浓度P = 0.0007，TNA浓度P = 0.0025）相比，随着时间推移观察到抗体滴度和浓度显著下降。首次血浆置换后总IgG浓度的下降速率（半衰期[t(1/2)] = 198.90天）显著低于抗PA IgG的下降速率（t(1/2) = 63.53天）（P < 0.0001），这表明抗PA IgG的减少更可能是由于自然衰减而非血浆置换。自上次注射后的时间以及首次血浆置换后的时间是考虑采集炭疽超免疫血浆最佳方案时的重要因素。针对PA的IgG与TNA抗体之间的良好相关性表明，抗PA酶联免疫吸附测定可作为供体血浆单位功能性免疫反应性的高通量筛查方法。
