Hagihara E, Itchoda N, Habu Y, Iida S, Mikami T, Kubo T
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, 060-8589, Japan.
Theor Appl Genet. 2005 Jul;111(2):250-5. doi: 10.1007/s00122-005-2010-z. Epub 2005 Jun 8.
We report here the molecular mapping of a fertility restorer gene (named Rf1) for Owen cytoplasmic male sterility in sugar beet. Eight AFLP and two RAPD markers, tightly linked to the Rf1 locus, were identified using bulked segregant analysis. Three AFLP markers, mAFEM972, mAFEM976 and mAFEM985, were found to co-segregate with the Rf1 allele in our mapping populations. With the help of RFLP markers, previously mapped on the sugar beet genome, we showed that Rf1 is positioned in the terminal region of linkage group Kiel III/Koeln IV. This map location agrees well with that found for the restorer gene X, which suggests that the Rf1 locus corresponds to the X locus. The availability of the molecular markers will facilitate the selection of maintainer-pollinator lines in breeding program and provide the foundation for map-based cloning of the Rf1 gene.
我们在此报告甜菜欧文细胞质雄性不育育性恢复基因（命名为Rf1）的分子定位。使用混合分离群体分析法鉴定出了八个与Rf1位点紧密连锁的AFLP标记和两个RAPD标记。在我们的定位群体中，发现三个AFLP标记mAFEM972、mAFEM976和mAFEM985与Rf1等位基因共分离。借助先前定位在甜菜基因组上的RFLP标记，我们表明Rf1位于连锁群基尔III/科隆IV的末端区域。该图谱位置与恢复基因X的位置非常吻合，这表明Rf1位点与X位点相对应。这些分子标记的可用性将有助于在育种计划中选择保持系授粉系，并为基于图谱的Rf1基因克隆提供基础。
Zotero 插件