Fernandez-Borja Mar, Janssen Lennert, Verwoerd Desiree, Hordijk Peter, Neefjes Jacques
The Netherlands Cancer Institute, Division of Tumour Biology, Amsterdam.
J Cell Sci. 2005 Jun 15;118(Pt 12):2661-70. doi: 10.1242/jcs.02384.
Rho GTPases are crucial regulators of the actin cytoskeleton and they play a role in the control of membrane trafficking. In contrast to the close family members RhoA and RhoC, RhoB localises to endosomes and delays epidermal growth factor receptor traffic. Here, we show that activated RhoB induces the peripheral distribution of endosomes, which align along subcortical actin stress fibres and are surrounded by an actin coat. The Diaphanous-related formin, Dia1, is recruited to endosomes by activated RhoB. Dia1 is required for the formation of the actin coat around endosomes downstream of RhoB, connecting membrane trafficking with the regulation of actin dynamics.
Rho GTP酶是肌动蛋白细胞骨架的关键调节因子，在膜运输控制中发挥作用。与紧密家族成员RhoA和RhoC不同，RhoB定位于内体并延迟表皮生长因子受体的运输。在此，我们表明活化的RhoB诱导内体的外周分布，这些内体沿着皮质下肌动蛋白应力纤维排列并被肌动蛋白外壳包围。Diaphanous相关的formin，即Dia1，被活化的RhoB招募到内体。Dia1是RhoB下游内体周围肌动蛋白外壳形成所必需的，它将膜运输与肌动蛋白动力学调节联系起来。
