Stone D L, Eatz R A, Rolib S D, Farlow S J, Hudson G S, Sinor L T
Immucor, Inc., PO Box 5625, Norcross, GA 30091, USA.
Immunohematology. 1990;6(1):12-7.
Recent technological advances in the immobilization and drying of red cell monolayers for use in solid phase red cell adherence (SPRCA) assays have resulted in the development of reagent red cells for antibody screening and identification that are stable at mom temperature. Panels consisting of twelve different RBC samples dried onto individual microplate wells were evaluated with 176 samples whose antibody specificities had previously been determined by conventional hemagglutination techniques. Identification tests performed with dried SPRCA panels proved to be more sensitive and less time consuming than hemagglutination tests. The red cell antigens of dried membranes were shown to be stable and reactive following storage for 120 days at mom temperature.
用于固相红细胞黏附（SPRCA）测定的红细胞单层固定和干燥技术的最新进展，已促成了用于抗体筛查和鉴定的试剂红细胞的开发，这些试剂红细胞在室温下稳定。对由干燥在单个微孔板孔上的12种不同红细胞样本组成的板，用176个样本进行了评估，这些样本的抗体特异性先前已通过传统血凝技术确定。用干燥的SPRCA板进行的鉴定试验被证明比血凝试验更灵敏且耗时更少。干燥膜的红细胞抗原在室温下储存120天后显示稳定且具有反应性。
