用重组可溶性CD4免疫的黑猩猩会产生具有抗人类免疫缺陷病毒活性的抗自身CD4抗体反应。
Chimpanzees immunized with recombinant soluble CD4 develop anti-self CD4 antibody responses with anti-human immunodeficiency virus activity.
作者信息
Watanabe M, Boyson J E, Lord C I, Letvin N L
机构信息
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
出版信息
Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5103-7. doi: 10.1073/pnas.89.11.5103.
Abstract
In view of the efficiency with which human immunodeficiency virus replication can be blocked in vitro with anti-CD4 antibodies, the elicitation of an anti-CD4 antibody response through active immunization might represent a useful therapeutic strategy for AIDS. Here we demonstrate that immunization of chimpanzees with recombinant soluble human CD4 elicited an anti-CD4 antibody response. The elicited antibody bound self CD4 on digitonin-treated but not freshly isolated lymphocytes. Nevertheless, this antibody blocked human immunodeficiency virus replication in chimpanzee and human lymphocytes. These observations suggest that immunization with recombinant soluble CD4 from human immunodeficiency virus-infected humans may be feasible and therapeutically beneficial.
摘要
鉴于抗CD4抗体在体外能够有效阻断人类免疫缺陷病毒的复制，通过主动免疫引发抗CD4抗体反应可能是一种治疗艾滋病的有效策略。在此，我们证明用重组可溶性人CD4免疫黑猩猩可引发抗CD4抗体反应。所引发的抗体可与经洋地黄皂苷处理的淋巴细胞上的自身CD4结合，但不能与新鲜分离的淋巴细胞上的自身CD4结合。然而，这种抗体可阻断黑猩猩和人类淋巴细胞中的人类免疫缺陷病毒复制。这些观察结果表明，用来自感染人类免疫缺陷病毒的人的重组可溶性CD4进行免疫可能是可行的，并且在治疗上有益。
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Immunization of simian immunodeficiency virus-infected rhesus monkeys with soluble human CD4 elicits an antiviral response.用可溶性人CD4免疫感染猿猴免疫缺陷病毒的恒河猴可引发抗病毒反应。
Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4616-20. doi: 10.1073/pnas.88.11.4616.
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Soluble human CD4 elicits an antibody response in rhesus monkeys that inhibits simian immunodeficiency virus replication.
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