Zentella A, Massagué J
Cell Biology and Genetics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5176-80. doi: 10.1073/pnas.89.11.5176.
Transforming growth factor beta 1 (TGF-beta 1) added to L6E9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the G1 phase of the cell cycle. This growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. These results suggest that TGF-beta 1 may act as a physiological inducer of myogenic differentiation in mitogen-rich environments, and its otherwise reversible growth inhibitory effect may become permanent if coupled to induction of terminal differentiation.
在富含促有丝分裂原的培养基中添加转化生长因子β1（TGF-β1）至L6E9大鼠骨骼肌成肌细胞，可导致c-myc表达迅速下降，并延迟细胞周期G1期的进程。这种生长抑制反应之后是细胞向终末分化的转变，伴有肌细胞生成素肌肉决定基因表达升高、肌肉特异性蛋白的诱导以及多核肌管的形成。这些结果表明，TGF-β1可能在富含促有丝分裂原的环境中作为肌源性分化的生理诱导剂，并且如果与终末分化的诱导相结合，其原本可逆的生长抑制作用可能会变得永久。
