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β型转化生长因子是肌源性分化的抑制剂。

Type beta transforming growth factor is an inhibitor of myogenic differentiation.

作者信息

Massagué J, Cheifetz S, Endo T, Nadal-Ginard B

出版信息

Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10. doi: 10.1073/pnas.83.21.8206.

DOI:10.1073/pnas.83.21.8206
PMID:3022285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC386896/
Abstract

We have investigated the effect of type beta transforming growth factor (TGF-beta) on the differentiation of skeletal muscle myoblasts. TGF-beta potently (ID50 approximately 10 pM) prevents established cell lines and primary cultures of rat and chicken embryo myoblasts from fusing into multinucleated myotubes. Inhibition of morphological differentiation by TGF-beta correlates with inhibition of the expression of muscle-specific mRNAs and proteins, strong induction of extracellular matrix type I collagen and fibronectin, and a marked tendency of the treated myoblasts to aggregate into densely multilayered arrays or clusters. Myogenic differentiation can resume after removal of TGF-beta from the medium. Examination of the time of action of TGF-beta shows that myoblasts stochastically reach a point beyond which they become insensitive to the inhibitory action of TGF-beta. This resistance of committed myoblasts to the inhibitory action of TGF-beta is not associated with any measurable change in the number or affinity of TGF-beta receptors in those cells. The results indicate that TGF-beta is a potent inhibitor of myogenesis and may regulate muscle development in vivo.

摘要

我们研究了β型转化生长因子（TGF-β）对骨骼肌成肌细胞分化的影响。TGF-β具有强大的作用（半数抑制浓度约为10 pM），可阻止已建立的细胞系以及大鼠和鸡胚成肌细胞的原代培养物融合形成多核肌管。TGF-β对形态分化的抑制与肌肉特异性mRNA和蛋白质表达的抑制、细胞外基质I型胶原和纤连蛋白的强烈诱导以及经处理的成肌细胞聚集成密集多层阵列或簇的明显趋势相关。从培养基中去除TGF-β后，肌源性分化可恢复。对TGF-β作用时间的研究表明，成肌细胞随机达到一个点，超过该点它们就对TGF-β的抑制作用变得不敏感。定向成肌细胞对TGF-β抑制作用的这种抗性与这些细胞中TGF-β受体数量或亲和力的任何可测量变化无关。结果表明，TGF-β是肌生成的有效抑制剂，可能在体内调节肌肉发育。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ed1/386896/7efd81a8f0da/pnas00325-0196-e.jpg
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