Pari Leelavinothan, Amali D Rosalin
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar, Tamil Nadu, India.
J Pharm Pharm Sci. 2005 Apr 30;8(1):115-23.
Tetrahydrocurcumin (THC) is an antioxidative substance, which is derived from curcumin, the component of turmeric. In the present investigation, the effect of THC and curcumin against chloroquine (CQ) induced hepatotoxicity were studied in female Wistar rats.
On single oral administration of CQ (970 mg/kg body weight) the activities of serum marker enzymes namely aspartate transaminase, alanine transaminase and alkaline phosphatase and the levels of bilirubin were significantly increased with significant alterations of lipids in serum and lipidperoxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides in plasma and liver were also elevated in CQ treated rats. The levels of non-enzymic antioxidants (vitamin C, vitamin E and reduced glutathione) and enzymic antioxidants (superoxide dismutase, catalase and glutathione peroxidase) were also decreased in CQ treated rats. Administration of THC (80 mg/kg body weight) and curcumin (80 mg/kg body weight) for 8 days before and 7 days after single administration of CQ significantly decreased the activities of serum markers and lipids in serum. In addition, the level of TBARS and hydroperoxides were significantly decreased with significant increase in non-enzymic and enzymic antioxidants on treatment with THC and curcumin. The biochemical observation was supplemented by histopathological examination of liver section. The results of the study reveal that THC shows more pronounced protective effect than curcumin against CQ induced toxicity.
四氢姜黄素（THC）是一种抗氧化物质，它源自姜黄的成分姜黄素。在本研究中，在雌性Wistar大鼠中研究了THC和姜黄素对氯喹（CQ）诱导的肝毒性的影响。
单次口服CQ（970毫克/千克体重）后，血清标志物酶即天冬氨酸转氨酶、丙氨酸转氨酶和碱性磷酸酶的活性以及胆红素水平显著升高，血清脂质也有显著改变，并且血浆和肝脏中脂质过氧化标志物如硫代巴比妥酸反应性物质（TBARS）和氢过氧化物也在经CQ处理的大鼠中升高。经CQ处理的大鼠中非酶抗氧化剂（维生素C、维生素E和还原型谷胱甘肽）和酶抗氧化剂（超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）的水平也降低。在单次给予CQ之前8天和之后7天给予THC（80毫克/千克体重）和姜黄素（80毫克/千克体重），显著降低了血清标志物的活性和血清中的脂质。此外，在用THC和姜黄素处理后，TBARS和氢过氧化物水平显著降低，非酶和酶抗氧化剂显著增加。通过肝脏切片的组织病理学检查对生化观察结果进行了补充。研究结果表明，THC对CQ诱导的毒性显示出比姜黄素更显著的保护作用。
