State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China.
Guangdong Institute for Drug Control, Guangzhou 510663, China.
Molecules. 2020 Mar 11;25(6):1278. doi: 10.3390/molecules25061278.
, a popular food and medicine dual purposes plant cultivated in tropical and subtropical regions, has been widely used as food crop and folk medicine, such as anti-diabetes agent, around the world. Triterpenoids have been considered as the major active ingredients of . In the present study, a high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC-DAD-ELSD) method was developed for simultaneous determination of nine triterpenoids in . Pressurized liquid extraction (PLE) was performed for sample preparation, and the analysis was achieved on a Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan) column eluted with gradient 0.1% aqueous formic acid-methanol system. The drift tube temperature of ELSD was set at 40 °C, and nitrogen flow-rate was at 1.6 L/min. All calibration curves for the analytes showed good linear regression ( > 0.9992) within test ranges. The established method was validated for intra-day and inter-day precisions (RSDs < 5%) and accuracy (recovery 94.23-106.87%). The validated method was successfully applied to determinate nine triterpenoids in 15 samples from the leave or fruit of P. guajava. In addition, the α-glucosidase inhibition assay showed good α-glucosidase inhibition activity in almost all the determined triterpenoids. The present study suggested that triterpenoids should be the quality control markers for and HPLC-DAD-ELSD was an effective tool for the quality control of .
番石榴，一种在热带和亚热带地区广泛种植的药食两用植物，已被广泛用作食品和民间药物，如抗糖尿病药物，在世界各地。三萜类化合物被认为是 的主要活性成分。本研究建立了一种高效液相色谱-二极管阵列-蒸发光散射检测（HPLC-DAD-ELSD）法，用于同时测定 中的 9 种三萜类化合物。加压液体萃取（PLE）用于样品制备，在 Cosmosil 5C18-MS-II（Nacalai Tesque，京都，日本）柱上以 0.1%甲酸-甲醇梯度洗脱系统进行分析。ELSD 的漂移管温度设定为 40°C，氮气流速为 1.6 L/min。所有分析物的校准曲线均表现出良好的线性回归（>0.9992）。所建立的方法在日内和日间精密度（RSDs<5%）和准确度（回收率 94.23-106.87%）方面均得到验证。该方法成功应用于测定 15 个番石榴叶或果实样品中的 9 种三萜类化合物。此外，α-葡萄糖苷酶抑制试验表明，几乎所有测定的三萜类化合物均具有良好的α-葡萄糖苷酶抑制活性。本研究表明，三萜类化合物应作为 的质量控制标志物，HPLC-DAD-ELSD 是 的质量控制的有效工具。
