Acosta-Gío A Enrique, Rueda-Patiño José L, Sánchez-Pérez Leonor
Microbiology and Infection Control Laboratory, Postgraduate Division, National University of Mexico Dental School, Mexico City, 04510 Mexico.
Am J Infect Control. 2005 Jun;33(5):307-9. doi: 10.1016/j.ajic.2004.10.005.
Various liquid chemical products are commercially available in Mexico under sporicidal label claims. Frequently, information provided on their labels conflicts with published data on active ingredients, use concentrations, or exposure times.
To evaluate sporicidal activity in 8 chemical products sold for the sterilization or high-level disinfection of medical and dental instruments.
Bacillus atrophaeus ATCC 9372 spores were suspended (10 6 spores in 50 mL) in each of 4 glutaraldehyde solutions: a super oxidized solution, a hydrogen peroxide formulation, a quaternary ammonium compound, and an ortho -phthalaldehyde solution. After up to a 10-hour exposure, the solutions were passed through 0.22-mum filters, which were then rinsed with 1% sodium bisulfite, washed, and incubated on tryptic soy agar for 5 days at 37 degrees C.
At the use concentrations stipulated on their labels, only 2 of 6 products registered as sterilants showed sporicidal activity when challenged with 6 log 10 : a 2% glutaraldehyde in 10 hours and the hydrogen peroxide solution in 6 hours. Of 2 products registered as high-level disinfectants, only the ortho -phthalaldehyde showed noticeable sporicidal activity after 10-hour exposure.
The results show that some chemical products, commercially available in Mexico as "Liquid Chemical Sterilants/High-Level Disinfectants" cannot be used reliably to process instruments between patients.
在墨西哥，各种液体化学产品在市场上销售，其标签声称具有杀孢子活性。通常，标签上提供的信息与已发表的关于活性成分、使用浓度或暴露时间的数据相冲突。
评估8种用于医疗和牙科器械灭菌或高水平消毒的化学产品的杀孢子活性。
将萎缩芽孢杆菌ATCC 9372孢子（50 mL中含10⁶个孢子）分别悬浮于4种戊二醛溶液中：一种超氧化溶液、一种过氧化氢制剂、一种季铵化合物和一种邻苯二甲醛溶液。暴露长达10小时后，将溶液通过0.22μm的过滤器，然后用1%亚硫酸氢钠冲洗、洗涤，并在37℃的胰蛋白胨大豆琼脂上培养5天。
在其标签规定的使用浓度下，6种注册为灭菌剂的产品中只有2种在受到6 log₁₀挑战时显示出杀孢子活性：10小时内的2%戊二醛溶液和6小时内的过氧化氢溶液。在2种注册为高水平消毒剂的产品中，只有邻苯二甲醛在暴露10小时后显示出明显的杀孢子活性。
结果表明，在墨西哥作为“液体化学灭菌剂/高水平消毒剂”销售的一些化学产品不能可靠地用于在患者之间处理器械。
