Kalinina Anna, Brown Daniel C W
Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada.
Plant Cell Rep. 2007 Jul;26(7):927-35. doi: 10.1007/s00299-007-0315-x. Epub 2007 Feb 24.
A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l(-1) 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l(-1 )ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.
已为9种观赏李属物种开发了一种微繁殖方法，这9种物种分别是美洲李、紫叶李、麦李、关山樱、月桂樱、山樱、毛樱桃、榆叶梅、‘舒伯特’稠李，它们在北美具有重要商业价值，还有常用于李属病毒检测的GF305桃。基于营养组织增殖的微繁殖周期包括通过在体外引入茎尖建立组织培养、芽增殖、生根诱导和植株驯化步骤，且可在5个月内完成。一种茎尖消毒方案可将细菌和真菌污染降至最低。通过使用1 mg l(-1)的6-苄基腺嘌呤，在观赏李属植物中获得了多芽形成。对于GF305桃，糖成分的改变，即用果糖代替蔗糖，并添加1 mg l(-1)的阿魏酸，对芽增殖率和长期体外培养的维持有显著影响。采用两步方案改善生根和植株驯化条件，即在含吲哚-3-丁酸（IBA）的培养基中进行4天的生根诱导，随后在不含IBA的培养基中进行3周的根伸长。将生根苗在基于蛭石的培养基中培养1个月，可使芽和根进一步生长，并提供更好的驯化和植株恢复效果。该微繁殖方法可用于保持李属植物的克隆特性，也可作为支持针对病毒感染的分生组织疗法的方案。
