Okajima Yasuhiko, Saika Shizuya, Sawa Mitsuru
Department of Ophthalmology, Nihon University School of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 2005 May;109(5):267-73.
In order to improve biocompatibility, we investigated the effects of surface modification by 2-methacryloyloxyethyl phosphorylcholine (MPC) on the foreign body reaction of intraocular lens (IOLs).
Materials of the IOLs were polymethylmethacrylate, hydrophobic acryl, and MPC surface-modified hydrophobic IOLs (MPC modified acryl). In an in vitro study, cultured macrophages sampled from mouse intra-abdominal exudate were cultured on a plate for each IOL material. The cell density and morphology of attached cells on the IOL materials were investigated. In an in vivo study, each IOL material was implanted in the peritoneal space of mice and foreign body reaction was investigated with a light microscope and a scanning electron microscope.
In the in vitro study, the cells on the MPC modified acryl IOL material were remarkably fewer than those on the plates of the other two IOL materials. Regarding the implanted IOL matrevials, MPC modified acryl IOL material showed more polynuclear giant foreign body cells in the early period than the other two IOL materials.
MPC surface modification can reduce the foreign body reaction of IOLs and has the potential to improve biocompatibility of IOL materials.
为了提高生物相容性，我们研究了用2-甲基丙烯酰氧乙基磷酰胆碱（MPC）进行表面改性对人工晶状体（IOL）异物反应的影响。
IOL的材料为聚甲基丙烯酸甲酯、疏水丙烯酸酯以及MPC表面改性的疏水IOL（MPC改性丙烯酸酯）。在一项体外研究中，从小鼠腹腔渗出液中采集的培养巨噬细胞在每种IOL材料的平板上培养。研究IOL材料上贴壁细胞的细胞密度和形态。在一项体内研究中，将每种IOL材料植入小鼠腹腔，并用光学显微镜和扫描电子显微镜研究异物反应。
在体外研究中，MPC改性丙烯酸酯IOL材料上的细胞明显少于其他两种IOL材料平板上的细胞。对于植入的IOL材料，MPC改性丙烯酸酯IOL材料在早期比其他两种IOL材料显示出更多的多核巨大异物细胞。
MPC表面改性可减少IOL的异物反应，并有可能提高IOL材料的生物相容性。
