Huang Xiao-Dan, Yao Ke, Zhang Hui, Huang Xiao-Jun, Xu Zhi-Kang
Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Clin Exp Ophthalmol. 2007 Jul;35(5):462-7. doi: 10.1111/j.1442-9071.2007.01516.x.
To analyse the in vitro adherence of Staphylococcus epidermidis to the 2-methacryloyl oxyethyl phosphorylcholine (MPC)-modified silicone intraocular lens (IOL).
The test IOLs were modified by using an air plasma treatment to bind MPC to the surface. The control IOLs were not modified. Chemical changes on the IOL surface were analysed by X-ray photoelectron spectroscopy (XPS) to confirm the covalent binding of MPC. IOL hydrophilicity was determined by measuring the water contact angle. Two different techniques, direct counting of viable adherent bacteria released by sonication, and scanning electron microscopy (SEM), were used to observe and compare the adherence of S. epidermidis to the IOLs after 1- and 18-h incubation.
XPS analysis confirmed that the test IOLs were surface-modified with MPC. The hydrophilicity of the IOLs was improved by surface modification, and the MPC-modified IOLs exhibited significantly reduced adhesion of S. epidermidis (P = 0.002) after an incubation period of 1 h. The SEM results showed that the MPC modification also suppressed the accumulation of bacteria and biofilm production after 18 h incubation.
MPC-modified hydrophilic silicone IOLs reduce bacterial adherence and colonization, and thus may help reduce the incidence of postoperative endophthalmitis.
分析表皮葡萄球菌对2-甲基丙烯酰氧乙基磷酰胆碱（MPC）修饰的硅水凝胶人工晶状体（IOL）的体外黏附情况。
通过空气等离子体处理将MPC结合到测试IOL表面进行修饰。对照IOL未进行修饰。采用X射线光电子能谱（XPS）分析IOL表面的化学变化，以确认MPC的共价结合。通过测量水接触角来测定IOL的亲水性。使用两种不同技术，即超声处理后对存活黏附细菌进行直接计数以及扫描电子显微镜（SEM），观察和比较表皮葡萄球菌在孵育1小时和18小时后对IOL的黏附情况。
XPS分析证实测试IOL表面用MPC进行了修饰。表面修饰提高了IOL的亲水性，并且MPC修饰的IOL在孵育1小时后表皮葡萄球菌的黏附显著降低（P = 0.002）。SEM结果表明，MPC修饰在孵育18小时后也抑制了细菌的积聚和生物膜形成。
MPC修饰的亲水性硅水凝胶IOL可减少细菌黏附和定植，因此可能有助于降低术后眼内炎的发生率。
