Higuma-Myojo Subaru, Sasaki Yasushi, Miyazaki Satomi, Sakai Masatoshi, Siozaki Arihiro, Miwa Naoko, Saito Shigeru
Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Toyama, Japan.
Am J Reprod Immunol. 2005 Jul;54(1):21-9. doi: 10.1111/j.1600-0897.2005.00279.x.
To examine whether the NK1/NK2/NK3/NKr1 paradigm can be adapted in natural killer (NK) cells.
Mononuclear cells were isolated from peripheral blood and/or decidua in healthy non-pregnant women (n = 17), early pregnant women (6-12 weeks of gestation, n = 17) and miscarriage cases (6-11 weeks of gestation, n = 10). We investigated the production of transforming growth factor (TGF)-beta, interleukin (IL)-4, IL-5, IL-10, IL-13, interferon (IFN)-gamma and tumor necrosis factor-alpha from peripheral blood- and decidual-CD56bright NK cells and -CD56dim NK cells by flow cytometry.
In the peripheral blood of the non-pregnant subjects, the main populations of CD56bright NK cells and CD56dim NK cells were IFN-gamma-producing NK1 type cells. Populations of IL-10-producing NKr1 type cells in peripheral blood CD56bright NK cells and CD56dim NK cells in early pregnant women were significantly greater compared with those in non-pregnant women, and these cells population decreased in miscarriage cases. In the early pregnancy decidua, the main populations of CD56bright NK cells and CD56dim NK cells were TGF-beta-producing NK3 type cells, and NK1 type cells were rare. NK3 type cells in decidua were significantly decreased in miscarriage cases compared with those in normal pregnant subjects. IL-4-, IL-5- or IL-13-producing NK2 type cells were rare in peripheral blood and decidua.
These data support the NK1/NK2/NK3/NKr1 hypothesis. NKr1 type cells in peripheral blood and NK3 type cells in decidua might play some important roles in the maintenance of pregnancy by regulation of maternal immune function.
研究自然杀伤（NK）细胞是否能适用NK1/NK2/NK3/NKr1模式。
从健康未孕女性（n = 17）、早孕女性（妊娠6 - 12周，n = 17）及流产病例（妊娠6 - 11周，n = 10）的外周血和/或蜕膜中分离单核细胞。我们通过流式细胞术研究外周血和蜕膜中CD56bright NK细胞及CD56dim NK细胞产生转化生长因子（TGF）-β、白细胞介素（IL）-4、IL-5、IL-10、IL-13、干扰素（IFN）-γ和肿瘤坏死因子-α的情况。
在未孕受试者的外周血中，CD56bright NK细胞和CD56dim NK细胞的主要群体是产生IFN-γ的NK1型细胞。早孕女性外周血CD56bright NK细胞和CD56dim NK细胞中产生IL-10的NKr1型细胞群体显著多于未孕女性，而这些细胞群体在流产病例中减少。在早孕蜕膜中，CD56bright NK细胞和CD56dim NK细胞的主要群体是产生TGF-β的NK3型细胞，NK1型细胞很少见。与正常妊娠受试者相比，流产病例蜕膜中的NK3型细胞显著减少。外周血和蜕膜中产生IL-4、IL-5或IL-13的NK2型细胞很少见。
这些数据支持NK1/NK2/NK3/NKr1假说。外周血中的NKr1型细胞和蜕膜中的NK3型细胞可能通过调节母体免疫功能在维持妊娠中发挥重要作用。
