Pallu Stéphane, Bourget Chantal, Bareille Reine, Labrugère Christine, Dard Michel, Sewing Andreas, Jonczyk Alfred, Vernizeau Michel, Christine Durrieu Marie, Amédée-Vilamitjana Joelle
INSERM, U577, Université Victor Segalen, Bordeaux, France.
Biomaterials. 2005 Dec;26(34):6932-40. doi: 10.1016/j.biomaterials.2005.04.054.
This study takes place in the field of development of a bioactive surface of titanium alloys. In this paper, titanium was functionalized with cyclo-DfKRG peptide by coating or grafting using different anchors (thiol or phosphonate) as spacers between the surface and the peptide. Cell adhesion, and differentiation of human osteoprogenitor (HOP) cells arising from human bone marrow were investigated. Our results seem to demonstrate that cyclo-DfKRG peptide coating with a phosphonate anchor and grafting procedure contributes to higher cell adhesion and a strong ALP and Cbfa1 mRNA expression, after 10 days of cell seeding. At the contrary, this peptide coated with a thiol anchor stimulates differentiation of HOP within 3 days of culture.
本研究发生在钛合金生物活性表面的开发领域。在本文中，通过使用不同的锚定基团（硫醇或膦酸酯）作为表面与肽之间的间隔物进行涂层或接枝，使钛用环-DfKRG肽进行功能化。研究了源自人骨髓的人骨祖细胞（HOP）的细胞粘附和分化。我们的结果似乎表明，在细胞接种10天后，用膦酸酯锚定基团进行环-DfKRG肽涂层和接枝程序有助于更高的细胞粘附以及强烈的碱性磷酸酶（ALP）和核心结合因子α1（Cbfa1）mRNA表达。相反，用硫醇锚定基团涂层的这种肽在培养3天内刺激HOP的分化。
