果胶涂层钛表面成骨细胞的分化

Differentiation of osteoblasts on pectin-coated titanium.

作者信息

Kokkonen H, Cassinelli C, Verhoef R, Morra M, Schols H A, Tuukkanen J

机构信息

Department of Anatomy and Cell Biology, University of Oulu, Post Office Box 5000, 90014 Oulu, Finland.

出版信息

Biomacromolecules. 2008 Sep;9(9):2369-76. doi: 10.1021/bm800356b. Epub 2008 Aug 5.

DOI:10.1021/bm800356b

PMID:18680340

Abstract

The gold standard for implant metals is titanium, and coatings such as collagen-I, RGD-peptide, chondroitin sulfate, and calcium phosphate have been used to modify its biocompatibility. We investigated how titanium coated with pectins, adaptable bioactive plant polysaccharides with anti-inflammatory effects, supports osteoblast differentiation. MC3T3-E1 cells, primary murine osteoblasts, and human mesenchymal cells (hMC) were cultured on titanium coated with rhamnogalacturonan-rich modified hairy regions (MHR-A and MHR-B) of apple pectin. Alkaline phosphatase (ALP) expression and activity, calcium deposition, and cell spreading were investigated. MHR-B, but not MHR-A, supports osteoblast differentiation. The MHR-A surface was not mineralized, but on MHR-B, the average mineralized area was 14.0% with MC3T3-E1 cells and 26.6% with primary osteoblasts. The ALP activity of hMCs on MHR-A was 58.3% at day 7 and 9.3% from that of MHR-B at day 10. These data indicate that modified pectin nanocoatings may enhance the biocompatibility of bone and dental implants.

摘要

植入金属的金标准是钛，胶原蛋白-I、RGD肽、硫酸软骨素和磷酸钙等涂层已被用于改善其生物相容性。我们研究了涂有果胶（一种具有抗炎作用的适应性生物活性植物多糖）的钛如何支持成骨细胞分化。将MC3T3-E1细胞、原代小鼠成骨细胞和人间充质细胞（hMC）培养在涂有富含鼠李糖半乳糖醛酸的苹果果胶修饰毛状区域（MHR-A和MHR-B）的钛上。研究了碱性磷酸酶（ALP）的表达和活性、钙沉积和细胞铺展情况。MHR-B支持成骨细胞分化，而MHR-A不支持。MHR-A表面未矿化，但在MHR-B上，MC3T3-E1细胞的平均矿化面积为14.0%，原代成骨细胞的平均矿化面积为26.6%。hMC在MHR-A上第7天的ALP活性为58.3%，在第10天为MHR-B的9.3%。这些数据表明，修饰的果胶纳米涂层可能会增强骨和牙科植入物的生物相容性。