Laboratory of Biomaterials, University of Geneva, Switzerland.
Clin Oral Implants Res. 2011 May;22(5):552-9. doi: 10.1111/j.1600-0501.2010.02033.x. Epub 2010 Nov 19.
Titanium is widely used in contemporary endosseous implantology and there is considerable thrust to further promote osseointegration by implant surface modifications. The aim of this study was to evaluate the effect of a titanium-nitride-oxide (TiNOx) coating on commercially pure microroughened titanium by assessing the proliferation and differentiation of human primary osteoblasts.
Cell proliferation, gene expression, alkaline phosphatase activity, osteoprotegerin and osteocalcin secretion were analyzed for a time course of 3 weeks, with or without additional stimulation by 1.25(OH)(2) vitamin D(3) 100 nM.
A 1.5-fold increase in the proliferation rate of cells grown on TiNOx-coated titanium as compared with uncoated surfaces was observed. SEM views indicated that the cells' normal morphology with their numerous extensions was maintained. The differentiation process on the TiNOx surface was only affected to a minor degree and translated into a slight delay in osteoblast maturation when compared to uncoated titanium.
Pending confirmation of these results in vivo, TiNOx coatings could potentially accelerate and enhance osseointegration.
钛广泛应用于当代的骨内种植体学，通过对种植体表面的改性来进一步促进骨整合是目前研究的热点。本研究的目的是通过评估人原代成骨细胞的增殖和分化来评价 TiNOx（氮化钛氧化物）涂层对商用纯微粗糙钛的影响。
分析细胞增殖、基因表达、碱性磷酸酶活性、护骨素和骨钙素分泌的时间过程为 3 周，有或没有额外的 1.25(OH)(2)维生素 D(3)100 nM 的刺激。
与未涂层表面相比，TiNOx 涂层钛上细胞的增殖速率增加了 1.5 倍。SEM 观察表明，细胞保持其正常形态和大量延伸。与未涂层钛相比，TiNOx 表面的分化过程仅受到轻微影响，导致成骨细胞成熟略有延迟。
在体内确认这些结果之前，TiNOx 涂层有可能加速和增强骨整合。
