Lessinger A C, Martins Junqueira A C, Lemos T A, Kemper E L, da Silva F R, Vettore A L, Arruda P, Azeredo-Espin A M
Laboratório de Genética Animal,Laboratório Genoma, Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Campinas, São Paulo, Brazil.
Insect Mol Biol. 2000 Oct;9(5):521-9. doi: 10.1046/j.1365-2583.2000.00215.x.
The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16,022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.
已确定嗜人锥蝇（Cochliomyia hominivorax）线粒体基因组的完整序列。该基因组大小为16,022 bp，对应于典型的短角亚目线粒体DNA。描述了COI的丝氨酸起始密码子以及COII、NADH 5和NADH 4基因的不完全终止密码子。嗜人锥蝇线粒体DNA的核苷酸组成富含77%的AT，这反映在蛋白质编码基因中富含AT的密码子占主导地位。在嗜人锥蝇线粒体基因中普遍观察到非最佳密码子使用情况。系统发育分析将无瓣蝇类物种作为一个单系群分布，并将嗜人锥蝇（有瓣蝇类）和无瓣蝇类聚集在一个典型的短角亚目簇中。讨论了在测序的线粒体基因组上诊断性限制性位点的鉴定及其与先前RFLP分析的相关性。
