Hungria M, Astolfi-Filho S, Chueire L M O, Nicolás M F, Santos E B P, Bulbol M R, Souza-Filho A, Nogueira Assunção E, Germano M G, Vasconcelos A T R
Embrapa Soja, Londrina, PR, Brazil.
Lett Appl Microbiol. 2005;41(1):17-23. doi: 10.1111/j.1472-765X.2005.01724.x.
To isolate and to characterize the diversity of Chromobacterium violaceum from the Brazilian Amazon region.
Twenty-two isolates were obtained from the waters and banks of the river Negro, in the Brazilian Amazon. All isolates were able to grow in vitro at 44 degrees C and pH 4.0, but were adversely affected by temperatures below 15 degrees C, and unable to survive at 4 degrees C, properties that may be related to the adaptation to the ecosystem. The isolates were joined at a final level of similarity of only 13% in the rep-PCR analysis. The analysis of 16S rRNA genes resulted in three main groups clustered at a final level of similarity of 97% and only three isolates were clustered with the type strain. Similar data were obtained for the 23S rRNA gene.
A high level of genetic diversity was verified with indications that the Brazilian isolates would fit into at least two new clusters besides C. violaceum species.
The results show remarkable bacterial adaptability and genetic diversity of C. violaceum in the Amazon region.
从巴西亚马逊地区分离并鉴定紫色色杆菌的多样性。
从巴西亚马逊地区内格罗河的水域和河岸获得了22株分离株。所有分离株都能够在44℃和pH 4.0条件下体外生长，但在低于15℃的温度下受到不利影响，并且在4℃下无法存活，这些特性可能与对生态系统的适应性有关。在重复PCR分析中，分离株最终的相似性水平仅为13%。16S rRNA基因分析产生了三个主要聚类，最终相似性水平为97%，只有三株分离株与模式菌株聚类。23S rRNA基因也获得了类似的数据。
证实了高水平的遗传多样性，表明除紫色色杆菌种外，巴西分离株至少可归入两个新的聚类。
结果显示了亚马逊地区紫色色杆菌显著的细菌适应性和遗传多样性。
