Muscariello Livio, Rosso Francesco, Marino Gerardo, Giordano Antonio, Barbarisi Manlio, Cafiero Gennaro, Barbarisi Alfonso
Department of Anaesthesological, Surgical and Emergency Sciences, IX Division of General Surgery and Applied Biotechnology, Second University of Naples, Naples, Italy.
J Cell Physiol. 2005 Dec;205(3):328-34. doi: 10.1002/jcp.20444.
Scanning Electron Microscope (SEM) is a powerful research tool, but since it requires high vacuum conditions, the wet materials and biological samples must undergo a complex preparation that limits the application of SEM on this kind of specimen and often causes the introduction of artifacts. The introduction of Environmental Scanning Electron Microscope (ESEM), working in gaseous atmosphere, represented a new perspective in biological research. Despite the fact that many biological applications have demonstrated the convenience of ESEM, the full potentialities of this technology are still under investigation. In this review, the exploration of the recent literature data confronted with the first results obtained in our experimental work suggest that ESEM represents an important extension of conventional scanning microscopy.
扫描电子显微镜（SEM）是一种强大的研究工具，但由于它需要高真空条件，湿材料和生物样品必须经过复杂的制备过程，这限制了SEM在这类样品上的应用，并且常常会引入伪像。环境扫描电子显微镜（ESEM）的出现，它可以在气态环境中工作，为生物学研究带来了新的视角。尽管许多生物学应用已经证明了ESEM的便利性，但这项技术的全部潜力仍在研究之中。在这篇综述中，对近期文献数据的探索以及与我们实验工作中获得的初步结果进行对比表明，ESEM是传统扫描显微镜的重要扩展。
