Shah Rucha, Mehta Dhoom Singh
Department of Periodontics, Bapuji Dental College and Hospital, Davangere, Karnataka, India.
J Indian Soc Periodontol. 2016 May-Jun;20(3):279-85. doi: 10.4103/0972-124X.179896.
Assess the prevalence of herpesviruses in healthy subjects, gingivitis, and chronic periodontitis patients, to assess the relationship between the prevalence of herpesviruses and periodontal clinical parameters, and to evaluate the effect of phase-I therapy on the level of viral detection.
Hundred patients consisting of 20 healthy subjects, 40 gingivitis, and 40 chronic periodontitis were included in the study. Clinical parameters recorded included plaque index, gingival index, sulcus bleeding index, probing depth, and clinical attachment level. The gingivitis and chronic periodontitis patients received phase-I periodontal therapy including oral hygiene instructions, full mouth scaling for gingivitis patients and scaling and root planing for chronic periodontitis patients. Gingival crevicular fluid (GCF) was collected, and the presence of herpes simplex virus-1 (HSV-1), HSV-2, cytomegalovirus, and Epstein-Barr virus (EBV) was analyzed using polymerase chain reaction (PCR). Recording of periodontal parameters as well as GCF collection was performed at baseline and 6 weeks postphase-I therapy.
At baseline, the levels of HSV-1 and EBV detection were lower in healthy controls as compared to gingivitis (P < 0.05) and chronic periodontitis cases (P < 0.001). Phase-I therapy led to reduction in the amount of HSV-1 and EBV in gingivitis patients (P < 0.05) and for HSV-1, human cytomegalovirus and EBV in chronic periodontitis patients (P < 0.05) in comparison to baseline. The prevalence of EBV in chronic periodontitis patients was positively associated with increased gingival index, probing depth and loss of clinical attachment (P < 0.05).
Higher prevalence of HSV-1 and EBV viruses in GCF of gingivitis and chronic periodontitis suggests a strong association between these viruses and periodontal diseases and periodontal therapy can lead to a reduction in herpesviruses at infected sites.
评估健康受试者、牙龈炎患者和慢性牙周炎患者中疱疹病毒的流行情况，评估疱疹病毒流行率与牙周临床参数之间的关系，并评估I期治疗对病毒检测水平的影响。
本研究纳入了100名患者，其中包括20名健康受试者、40名牙龈炎患者和40名慢性牙周炎患者。记录的临床参数包括菌斑指数、牙龈指数、龈沟出血指数、探诊深度和临床附着水平。牙龈炎和慢性牙周炎患者接受I期牙周治疗，包括口腔卫生指导，牙龈炎患者进行全口洁治，慢性牙周炎患者进行龈下刮治和根面平整。收集龈沟液（GCF），并使用聚合酶链反应（PCR）分析单纯疱疹病毒1型（HSV-1）、HSV-2、巨细胞病毒和爱泼斯坦-巴尔病毒（EBV）的存在情况。在基线和I期治疗后6周记录牙周参数并收集GCF。
在基线时，与牙龈炎患者（P < 0.05）和慢性牙周炎患者（P < 0.001）相比，健康对照组中HSV-1和EBV的检测水平较低。与基线相比，I期治疗使牙龈炎患者的HSV-1和EBV数量减少（P < 0.05），慢性牙周炎患者的HSV-1、人巨细胞病毒和EBV数量减少（P < 0.05）。慢性牙周炎患者中EBV的流行率与牙龈指数增加、探诊深度增加和临床附着丧失呈正相关（P < 0.05）。
牙龈炎和慢性牙周炎患者的GCF中HSV-1和EBV病毒的流行率较高，表明这些病毒与牙周疾病之间存在密切关联，牙周治疗可导致感染部位的疱疹病毒减少。
