Elek János, Mangelings Debby, Iványi Timea, Lázár István, Heyden Y Vander
Research Group of Homogeneous Catalysis Hungarian Academy of Sciences, University of Debrecen, P.O. Box 7, Debrecen H-4010, Hungary.
J Pharm Biomed Anal. 2005 Jul 15;38(4):601-8. doi: 10.1016/j.jpba.2005.02.014. Epub 2005 Apr 7.
Different dual selector systems containing a cyclodextrin derivative (methyl-beta-cyclodextrin and dimethyl-beta-cyclodextrin) and a new diaza-crown-ether derivative (N-[2-(1,4,10,13-tetraoxa-7,16-diazacyclooctadecan-7-yl)propanoyl]glycine) were studied in the enantioselective separation of tryptophan-methylester and tyrosine-methylester enantiomers. This paper deals with the systematic study of the effects of changing the composition of the background electrolyte on the resolution of the d- and l- forms using an experimental design approach. It was found that the dual systems allowed a better chiral separation of the amino acid derivatives. The experimental design approach also allowed improving the separation compared to the starting conditions (center point of the design), which were adopted from a previous study.
