Xu Ning, Xu Ming, Zhang You-Yi
Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, China.
Sheng Li Xue Bao. 2005 Jun 25;57(3):271-7.
Single molecule detection is a technology of studying biomolecules with high spatial and temporal resolution. By exploiting recent technical advances, we are able to observe, detect, even manipulate individual molecules and study their conformational changes and dynamic behaviors. New information can be obtained from the single molecule research, which is otherwise hidden or averaged out. In recent years, the development of single molecule detection techniques has opened up a new era of life science. In this review, we introduce the advances of the techniques for detecting single molecules in cell biology and review the development of single molecule detection in living cells.
单分子检测是一种以高空间和时间分辨率研究生物分子的技术。通过利用最近的技术进展，我们能够观察、检测甚至操纵单个分子，并研究它们的构象变化和动态行为。单分子研究可以获得新的信息，这些信息在其他情况下会被隐藏或平均化。近年来，单分子检测技术的发展开启了生命科学的新纪元。在这篇综述中，我们介绍了细胞生物学中检测单分子技术的进展，并回顾了活细胞中单分子检测的发展情况。
