Hoffmann R A, Geijtenbeek T, Kamerling J P, Vliegenthart J F
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Carbohydr Res. 1992 Jan;223:19-44. doi: 10.1016/0008-6215(92)80003-j.
The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1----4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, ----4)[alpha-L-Araf-(1----3)]-beta-D-Xylp-(1---- and/or ----4) [alpha-L-Araf-(1----2)][alpha-L-Araf-(1----3)]-beta-D-Xyl p-(1----, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established.
通过用内切（1→4）-β-D-木聚糖酶消化小麦胚乳阿拉伯木聚糖生成、并经Bio-Gel P-6凝胶渗透色谱和带脉冲安培检测的高效阴离子交换色谱（h.p.a.e.-p.a.d.）分离得到的七糖至十四糖的结构，使用单糖和甲基化分析、快原子轰击质谱（f.a.b.-m.s.）和1H-核磁共振光谱进行了阐明。所鉴定的结构有两个分支单元，即→4)[α-L-阿拉伯呋喃糖基-(1→3)]-β-D-木糖基-(1→和/或→4)[α-L-阿拉伯呋喃糖基-(1→2)][α-L-阿拉伯呋喃糖基-(1→3)]-β-D-木糖基-(1→，它们以所有四种可能的组合彼此直接相连。h.p.a.e.-p.a.d.洗脱模式表明这些组合的含量并不相等。此外，还发现了含有两个由一个或两个无分支β-D-木糖基残基隔开的2,3-分支β-D-木糖基残基的化合物，并且确定了一种含有三个2,3-分支β-D-木糖基残基的十四糖的存在。
