Huang Ling, Nie Wenhui, Wang Jinhuan, Su Weiting, Yang Fengtang
Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, 650223, PR China.
Chromosome Res. 2005;13(4):389-99. doi: 10.1007/s10577-005-0977-6.
Chromosomal homologies have been established between the Chinese muntjac (Muntiacus reevesi, MRE, 2n = 46) and five ovine species: wild goat (Capra aegagrus, CAE, 2n = 60), argali (Ovis ammon, OAM, 2n = 56), snow sheep (Ovis nivicola, ONI, 2n = 52), red goral (Naemorhedus cranbrooki, NCR, 2n = 56) and Sumatra serow (Capricornis sumatraensis, CSU, 2n = 48) by chromosome painting with a set of chromosome-specific probes of the Chinese muntjac. In total, twenty-two Chinese muntjac autosomal painting probes detected thirty-five homologous segments in the genome of each species. The chromosome X probe hybridized to the whole X chromosomes of all ovine species while the chromosome Y probe gave no signal. Our results demonstrate that almost all homologous segments defined by comparative painting show a high degree of conservation in G-banding patterns and that each speciation event is accompanied by specific chromosomal rearrangements. The combined analysis of our results and previous cytogenetic and molecular systematic results enables us to map the chromosomal rearrangements onto a phylogenetic tree, thus providing new insights into the karyotypic evolution of these species.
通过使用一组中华麂的染色体特异性探针进行染色体涂染，已经确定了中华麂（Muntiacus reevesi，MRE，2n = 46）与五种绵羊物种之间的染色体同源性：野山羊（Capra aegagrus，CAE，2n = 60）、盘羊（Ovis ammon，OAM，2n = 56）、雪羊（Ovis nivicola，ONI，2n = 52）、赤斑羚（Naemorhedus cranbrooki，NCR，2n = 56）和苏门羚（Capricornis sumatraensis，CSU，2n = 48）。总共，22个中华麂常染色体涂染探针在每个物种的基因组中检测到35个同源片段。X染色体探针与所有绵羊物种的整个X染色体杂交，而Y染色体探针未给出信号。我们的结果表明，通过比较涂染定义的几乎所有同源片段在G带模式上都显示出高度保守性，并且每个物种形成事件都伴随着特定的染色体重排。对我们的结果与先前的细胞遗传学和分子系统学结果进行综合分析，使我们能够将染色体重排映射到系统发育树上，从而为这些物种的核型进化提供新的见解。
