Yang F, Müller S, Just R, Ferguson-Smith M A, Wienberg J
Department of Pathology, University of Cambridge, England.
Genomics. 1997 Feb 1;39(3):396-401. doi: 10.1006/geno.1996.4497.
We have used human chromosome-specific painting probes for in situ hybridization on Indian muntjac (Muntiacus muntjak vaginalis, 2n = 6, 7) metaphase chromosomes to identify the homologous chromosome regions of the entire human chromosome set. Chromosome rearrangements that have been involved in the karyotype evolution of these two species belonging to different mammalian orders were reconstructed based on hybridization patterns. Although, compared to human chromosomes, the karyotype of the Indian muntjac seems to be highly rearranged, we could identify a limited number of highly conserved homologous chromosome regions for each of the human chromosome-specific probes. We identified 48 homologous autosomal chromosome segments, which is in the range of the numbers found in other artiodactyls and carnivores recently analyzed by chromosome painting. The results demonstrate that the reshuffling of the muntjac karyotype is mostly due to fusions of huge blocks of entire chromosomes. This is in accordance with previous chromosome painting analyses between various Muntjac species and contrasts the findings for some other mammals (e.g., gibbons, mice) that show exceptional chromosome reshuffling due to multiple reciprocal translocation events.
我们使用人类染色体特异性涂染探针，对印度麂（赤麂，2n = 6, 7）中期染色体进行原位杂交，以鉴定整个人类染色体组的同源染色体区域。基于杂交模式，重建了涉及这两个属于不同哺乳纲目的物种核型进化的染色体重排。尽管与人类染色体相比，印度麂的核型似乎高度重排，但我们能够为每个人类染色体特异性探针鉴定出有限数量的高度保守的同源染色体区域。我们鉴定出48个同源常染色体片段，这在最近通过染色体涂染分析的其他偶蹄目动物和食肉动物中发现的数量范围内。结果表明，麂核型的重排主要是由于整条染色体的大片段融合。这与之前对各种麂属物种之间的染色体涂染分析结果一致，并且与一些其他哺乳动物（如长臂猿、小鼠）的研究结果形成对比，这些哺乳动物由于多次相互易位事件而表现出异常的染色体重排。
