DnaE蛋白（DNA聚合酶III的α亚基）的过量产生可恢复在DNA聚合酶I缺陷的条件性非存活大肠杆菌菌株中的生存能力。
Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.
作者信息
Witkin E M, Roegner-Maniscalco V
机构信息
Waksman Institute, Rutgers State University of New Jersey, Piscataway, New Jersey 08854.
出版信息
J Bacteriol. 1992 Jun;174(12):4166-8. doi: 10.1128/jb.174.12.4166-4168.1992.
Abstract
A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication and cell viability, as well as the PolI-dependent replication of the plasmid carrying dnaE.
摘要
大肠杆菌的polA12 recA718双突变体中，DNA聚合酶I对温度敏感，在42℃时无法维持正常的DNA合成，也不能在丰富培养基上形成菌落。DNA聚合酶III的聚合α亚基DnaE蛋白的过量表达恢复了细菌的DNA复制和细胞活力，以及携带dnaE的质粒的依赖PolI的复制。
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