SAFOSO, Bremgartenstrasse, Bern, Switzerland.
Zoonoses Public Health. 2010 Dec;57(7-8):e130-5. doi: 10.1111/j.1863-2378.2009.01299.x.
Trichinellosis is a food-borne zoonotic disease caused by the nematode Trichinella spp. Many omnivorous and carnivorous animal species can act as host for this parasite, including domestic pigs. To protect public health, it should be ensured that pork should not contain infective Trichinella larvae. Surveillance for Trichinella spp. can be done using direct (larval detection) and indirect (antibody detection) diagnostic techniques. The aim of this study was to demonstrate the absence of infection in Swiss domestic pigs. An ELISA was used as the initial screening test, and sera reacting in ELISA were further investigated using both a Western blot for serology and an artificial digestion test with 20 g of diaphragm tissue for larval detection. A total of 7412 adult pigs, 9973 finishing pigs and 2779 free-ranging pigs were tested. Samples from 17 (0.23%) adult pigs, 16 (0.16%) finishing pigs and nine (0.32%) free-ranging pigs were ELISA-positive, but all of these sera were subsequently negative by Western blot and by the artificial digestion method. Based on these findings, an absence of Trichinella infections in adult pigs (target prevalence 0.04%) and finishing pigs (target prevalence 0.03%) can be concluded. The results also demonstrated that the prevalence of Trichinella infections does not exceed 0.11% in free-ranging pigs, the group with the highest risk of exposure.
旋毛虫病是一种食源性人畜共患疾病，由旋毛线虫引起。许多杂食性和肉食性动物都可以成为这种寄生虫的宿主，包括家猪。为了保护公众健康，应确保猪肉不含有感染性旋毛虫幼虫。旋毛虫的监测可采用直接（幼虫检测）和间接（抗体检测）诊断技术。本研究旨在证明瑞士家猪无感染。采用 ELISA 作为初始筛选试验，对 ELISA 反应阳性的血清进一步采用 Western blot 进行血清学检测，以及采用 20 克膈肌组织进行人工消化试验进行幼虫检测。共检测了 7412 头成年猪、9973 头育肥猪和 2779 头自由放养猪。17 头（0.23%）成年猪、16 头（0.16%）育肥猪和 9 头（0.32%）自由放养猪的血清 ELISA 检测呈阳性，但所有这些血清随后通过 Western blot 和人工消化方法检测均呈阴性。基于这些发现，可以得出成年猪（目标流行率为 0.04%）和育肥猪（目标流行率为 0.03%）无旋毛虫感染的结论。结果还表明，在风险最高的自由放养猪中，旋毛虫感染的流行率不超过 0.11%。
