Makino S-I, Kawamoto K, Takeshi K, Okada Y, Yamasaki M, Yamamoto S, Igimi S
Laboratory of Food Microbiology and Immunology, Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Int J Food Microbiol. 2005 Oct 15;104(2):189-96. doi: 10.1016/j.ijfoodmicro.2005.02.009.
Food-borne outbreaks caused by Listeria monocytogenes have been recognized in US and European countries. Only sporadic cases, of neonatal listeriosis, have been reported in Japan. Since L. monocytogenes has been often isolated from foods in Japan, food-borne outbreaks potentially could have occurred. In February 2001, L. monocytogenes serotype 1/2b was isolated from a washed-type cheese during routine Listeria monitoring of 123 domestic cheeses. Further samples from products and the environments at the plant that produced the contaminated cheese were examined for L. monocytogenes. L. monocytogenes serotype 1/2b was detected in 15 cheese samples, at most probable number that ranged from <30 to 4.6 x 10(9)/100 g, and in environmental samples. Studies with people who had consumed cheese from the plant revealed 86 persons who had been infected with L. monocytogenes. Thirty-eight of those people had developed clinical symptoms of gastroenteritis or the common cold type after the consumption of cheese. Isolates from those patients exhibited the same serotype, pathogenicity for mice and HeLa cells, DNA fingerprinting patterns and PCR amplification patterns. From the epidemiological and genetic evidence, it appeared that the outbreak was caused by cheese. This is the first documented incidence of food-borne listeriosis in Japan.
在美国和欧洲国家，已确认由单核细胞增生李斯特菌引起食源性疾病暴发。在日本，仅报告过新生儿李斯特菌病的散发病例。由于在日本经常从食品中分离出单核细胞增生李斯特菌，因此有可能发生食源性疾病暴发。2001年2月，在对123种国产奶酪进行李斯特菌常规监测期间，从一种水洗型奶酪中分离出1/2b血清型单核细胞增生李斯特菌。对生产受污染奶酪的工厂的产品和环境中的进一步样本进行了单核细胞增生李斯特菌检测。在15份奶酪样本中检测到1/2b血清型单核细胞增生李斯特菌，其最大可能数范围为<30至4.6×10⁹/100g，环境样本中也检测到。对食用了该工厂奶酪的人群进行的研究发现，有86人感染了单核细胞增生李斯特菌。其中38人在食用奶酪后出现了肠胃炎或普通感冒型的临床症状。从这些患者身上分离出的菌株表现出相同的血清型、对小鼠和HeLa细胞的致病性、DNA指纹图谱和PCR扩增图谱。从流行病学和遗传学证据来看，此次暴发似乎是由奶酪引起的。这是日本有记录的首例食源性李斯特菌病。
