Moser Jennifer M, Freitas Tori, Arasu Prema, Gibson Greg
Department of Genetics, North Carolina State University, Raleigh, NC 27695, USA.
Mol Biochem Parasitol. 2005 Sep;143(1):39-48. doi: 10.1016/j.molbiopara.2005.04.012.
Ancylostoma caninum is a common canine parasite responsible for anemia and death in infected dogs. Gene expression profiling was used to investigate molecular differences between two different forms of the third larval stage (L3s): infective free-living larvae and in vitro serum-stimulated larvae that mimic the initial stages of parasitism of a host. We developed an A. caninum cDNA microarray consisting of 4191 EST clones, and used it to identify a set of 113 genes that are differentially regulated between infective and parasitic larval stages. Real-time RT-PCR was used to confirm the expression differences of a subset of the genes. Of the genes repressed upon serum stimulation, seven encode members of the 'Ancylostoma secreted protein' ASP family, while another transcript encoding a 24 kDa excretory protein with similarity to ASP was up-regulated in serum-stimulated L3s. This suggests that different members of a protein family that has important implications for the hookworm's parasitic lifestyle are regulated in a complementary manner in response to serum stimulation. Comparison of two strains of A. caninum from North Carolina and Maryland only identified a single gene, one of the members of the ASP family, that was differentially repressed upon serum stimulation.
犬钩虫是一种常见的犬类寄生虫，可导致受感染犬只贫血和死亡。基因表达谱分析用于研究第三期幼虫（L3）两种不同形态之间的分子差异：感染性自由生活幼虫和模拟宿主寄生初期的体外血清刺激幼虫。我们开发了一种由4191个EST克隆组成的犬钩虫cDNA微阵列，并用它来鉴定一组在感染性幼虫阶段和寄生性幼虫阶段之间差异调节的113个基因。实时RT-PCR用于确认部分基因的表达差异。在血清刺激后被抑制的基因中，有7个编码“犬钩虫分泌蛋白”（ASP）家族成员，而另一个与ASP相似的编码24 kDa排泄蛋白的转录本在血清刺激的L3中上调。这表明，对钩虫寄生生活方式具有重要意义的一个蛋白家族的不同成员在对血清刺激的反应中以互补方式受到调节。对来自北卡罗来纳州和马里兰州的两株犬钩虫进行比较，仅鉴定出一个基因，即ASP家族成员之一，该基因在血清刺激后被差异抑制。
