Ikeda Yuko, Park Enock Y, Okuda Naoyuki
Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
Bioresour Technol. 2006 May;97(8):1030-5. doi: 10.1016/j.biortech.2005.04.040. Epub 2005 Jun 24.
Gluconic acid production was investigated using an enzymatic hydrolysate of waste office automation paper in a culture of Aspergillus niger. In repeated batch cultures using flasks, saccharified solution medium (SM) did not show any inhibitory effects on gluconic acid production compared to glucose medium (GM). The average gluconic acid yields were 92% (SM) and 80% (GM). In repeated batch cultures using SM in a turbine blade reactor (TBR), the gluconic acid yields were 60% (SM) and 67% (GM) with 80-100 g/l of gluconic acid. When pure oxygen was supplied the production rate increased to four times higher than when supplying air. Remarkable differences in the morphology of A. niger and dry cell weight between SM and GM were observed. The difference in morphology may have caused a reduction of oxygen transfer, resulting in a decrease in gluconic acid production rate in SM.
利用废弃办公自动化纸张的酶解产物在黑曲霉培养物中研究了葡萄糖酸的生产。在使用烧瓶的重复分批培养中,与葡萄糖培养基(GM)相比,糖化溶液培养基(SM)对葡萄糖酸的生产没有显示出任何抑制作用。葡萄糖酸的平均产率分别为92%(SM)和80%(GM)。在涡轮叶片反应器(TBR)中使用SM进行重复分批培养时,葡萄糖酸产率分别为60%(SM)和67%(GM),葡萄糖酸浓度为80 - 100 g/l。当供应纯氧时,生产率提高到供应空气时的四倍。观察到SM和GM之间黑曲霉形态和干细胞重量存在显著差异。形态上的差异可能导致了氧传递的减少,从而导致SM中葡萄糖酸生产率的降低。