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本文引用的文献

1
Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria.优化蛋白表达载体和分泌信号肽以驱动乳酸菌中细菌素肠菌素 A 的生产、分泌和功能表达。
J Biotechnol. 2011 Oct 20;156(1):76-86. doi: 10.1016/j.jbiotec.2011.07.038. Epub 2011 Aug 4.
2
Are bacteriocins underexploited? Novel applications for old antimicrobials.细菌素是否未被充分利用？新型抗菌药物的新应用。
Curr Pharm Biotechnol. 2011 Aug;12(8):1205-20. doi: 10.2174/138920111796117364.
3
Use of the usp45 lactococcal secretion signal sequence to drive the secretion and functional expression of enterococcal bacteriocins in Lactococcus lactis.利用乳球菌的 usp45 分泌信号序列驱动肠球菌细菌素在乳球菌中的分泌和功能表达。
Appl Microbiol Biotechnol. 2011 Jan;89(1):131-43. doi: 10.1007/s00253-010-2849-z. Epub 2010 Sep 15.
4
Towards universal systems for recombinant gene expression.朝着重组基因表达的通用系统发展。
Microb Cell Fact. 2010 Apr 30;9:27. doi: 10.1186/1475-2859-9-27.
5
Enterocins in food preservation.食品保鲜中的肠菌素。
Int J Food Microbiol. 2010 Jun 30;141(1-2):1-10. doi: 10.1016/j.ijfoodmicro.2010.03.005. Epub 2010 Mar 11.
6
Use of the yeast Pichia pastoris as an expression host for secretion of enterocin L50, a leaderless two-peptide (L50A and L50B) bacteriocin from Enterococcus faecium L50.利用酵母毕赤酵母作为表达宿主，分泌来自屎肠球菌 L50 的无信号肽的双肽（L50A 和 L50B）细菌素 enterocin L50。
Appl Environ Microbiol. 2010 May;76(10):3314-24. doi: 10.1128/AEM.02206-09. Epub 2010 Mar 26.
7
Bacterial systems for production of heterologous proteins.用于生产异源蛋白的细菌系统。
Methods Enzymol. 2009;463:149-68. doi: 10.1016/S0076-6879(09)63012-3.
8
Xplor 2--an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans.Xplor 2——一种用于在嗜腺嘌呤阿苏酵母中生产重组蛋白的优化转化/表达系统。
Appl Microbiol Biotechnol. 2009 Sep;84(3):583-94. doi: 10.1007/s00253-009-2167-5. Epub 2009 Aug 12.
9
Atan1p-an extracellular tannase from the dimorphic yeast Arxula adeninivorans: molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme.Atan1p——来自双态酵母嗜腺嘌呤阿苏菌的一种胞外单宁酶：ATAN1基因的分子克隆及重组酶的特性分析
Yeast. 2009 Jun;26(6):323-37. doi: 10.1002/yea.1669.
10
Development of bacteriocinogenic strains of Saccharomyces cerevisiae heterologously expressing and secreting the leaderless enterocin L50 peptides L50A and L50B from Enterococcus faecium L50.酿酒酵母产细菌素菌株的开发，该菌株可异源表达并分泌来自粪肠球菌L50的无前导肽肠球菌素L50肽L50A和L50B。
Appl Environ Microbiol. 2009 Apr;75(8):2382-92. doi: 10.1128/AEM.01476-08. Epub 2009 Feb 13.

通过毕赤酵母、乳酸克鲁维酵母、汉逊酵母和腺嘌呤缺陷型酒香酵母对肠球菌 T136 产生的细菌素肠菌素 A 进行克隆、生产和功能表达。

Cloning, production, and functional expression of the bacteriocin enterocin A, produced by Enterococcus faecium T136, by the yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans.

机构信息

Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid (UCM), Madrid, Spain.

出版信息

Appl Environ Microbiol. 2012 Aug;78(16):5956-61. doi: 10.1128/AEM.00530-12. Epub 2012 Jun 8.

DOI:10.1128/AEM.00530-12
PMID:22685156
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3406115/
Abstract

The bacteriocin enterocin A (EntA) produced by Enterococcus faecium T136 has been successfully cloned and produced by the yeasts Pichia pastoris X-33EA, Kluyveromyces lactis GG799EA, Hansenula polymorpha KL8-1EA, and Arxula adeninivorans G1212EA. Moreover, P. pastoris X-33EA and K. lactis GG799EA produced EntA in larger amounts and with higher antimicrobial and specific antimicrobial activities than the EntA produced by E. faecium T136.

摘要

由屎肠球菌 T136 产生的细菌素肠菌素 A (EntA) 已被成功地通过酵母毕赤酵母 X-33EA、乳酸克鲁维酵母 GG799EA、汉逊酵母 KL8-1EA 和腺嘌呤营养酵母 G1212EA 进行克隆和生产。此外，与屎肠球菌 T136 产生的 EntA 相比，毕赤酵母 X-33EA 和乳酸克鲁维酵母 GG799EA 产生的 EntA 具有更高的抗菌和特定抗菌活性，且产生量更大。