Sale Ken, Song Likai, Liu Yi-Shiuan, Perozo Eduardo, Fajer Piotr
Biosystems Research Department, Sandia National Laboratories, Livermore, California, USA.
J Am Chem Soc. 2005 Jul 6;127(26):9334-5. doi: 10.1021/ja051652w.
Current SDSL-EPR methods allow measurement of dipolar distances in the 8-70 A range; however, the use of extrinsic probes complicates the interpretation of these distances in modeling macromolecular structure and conformational changes. The data presented here show that interprobe distances correlate only weakly with Cbeta-Cbeta distances, especially for distances that are on the order of the spin label tether lengths. Explicitly incorporating the spin label into the modeling process increases the experiment/model correlation 4-fold and reduces the distance error from 6 A to 3 A.
当前的单对双电子自旋共振（SDSL-EPR）方法能够测量8至70埃范围内的偶极距离；然而，使用外在探针会使在构建大分子结构和构象变化模型时对这些距离的解释变得复杂。此处呈现的数据表明，探针间距离与Cβ - Cβ距离的相关性较弱，尤其是对于与自旋标记连接链长度相当的距离。将自旋标记明确纳入建模过程可使实验/模型相关性提高4倍，并将距离误差从6埃降至3埃。
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