Marucci G, La Rosa G, Pozio E
Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
J Helminthol. 2010 Sep;84(3):336-9. doi: 10.1017/S0022149X10000131. Epub 2010 Mar 16.
Molecular analyses such as polymerase chain reaction (PCR) and sequencing are very useful for taxon identification, especially when morphological characters useful for identifying taxa are lacking. However, the use of molecular tools can be the source of taxon misidentification if they are not correctly applied and the results are not critically evaluated and compared with the literature and GenBank data. We describe a case of misidentification of a taxon of the genus Trichinella due to sequencing mistakes, lack of reference material and selection of a single molecular marker. A Trichinella sp. isolate from an Iranian wild boar (Sus scrofa) was identified as belonging to the Nearctic species Trichinella murrelli, through the molecular analysis of the 5S rRNA intergenic spacer region. A successive molecular identification of the same isolate was performed by the International Trichinella Reference Centre in Rome, Italy, using the 5S rRNA intergenic spacer region, the LSU rDNA expansion segment five, and the internal transcribed spacers 1 and 2. According to these analyses, the Iranian isolate belonged to Trichinella britovi, a Palaearctic species already described in Iran.
诸如聚合酶链反应（PCR）和测序等分子分析方法对于分类单元的鉴定非常有用，尤其是在缺乏用于鉴定分类单元的形态特征时。然而，如果分子工具使用不当，且结果未经过严格评估并与文献及基因库数据进行比较，那么这些工具可能会导致分类单元误判。我们描述了一例因测序错误、缺乏参考材料以及选择单一分子标记而导致旋毛虫属一个分类单元误判的案例。通过对5S rRNA基因间隔区进行分子分析，从一头伊朗野猪（野猪）分离出的旋毛虫属物种被鉴定为属于新北区物种穆氏旋毛虫。意大利罗马的国际旋毛虫参考中心使用5S rRNA基因间隔区、大亚基核糖体DNA扩展片段5以及内转录间隔区1和2对同一分离株进行了后续分子鉴定。根据这些分析，该伊朗分离株属于布氏旋毛虫，这是一种已在伊朗被描述过的古北区物种。
