Stojanović Natasa, Rogić Dunja, Stavljenić-Rukavina Ana
Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
Clin Chem Lab Med. 2005;43(6):646-53. doi: 10.1515/CCLM.2005.111.
The Konelab 20XT (Thermo Electron Oy, Finland) is a clinical chemistry analyzer for colorimetric, immunoturbidimetric and ion-selective electrode measurements. The aim of our work was to evaluate the analytical performances of the Konelab 20XT according to the European Clinical Chemistry Laboratory Standards Guidelines. A total of 30 analytes including substrates, enzymes, electrolytes and specific proteins were tested. Investigation results showed low imprecision (within-run coefficient of variation was below 3.5% and between-day coefficient of variation was less than 2.5% for most analytes at all three levels studied) and acceptable accuracy of the analyzer. No significant sample- or reagent-related carry-over was found. It was demonstrated that the analytical system operates within the claimed linearity ranges. The results compared well with those obtained by instruments routinely used in our laboratory (Olympus AU2700, Behring Nephelometer II). In general, the data on interference by hemoglobin, hyperbilirubinemia and turbidity are in accordance with known facts. However, slight hemolysis was found to interfere with the alkaline phosphatase (ALP) assay and mild lipemia affected the glucose assay. The Konelab 20XT is an easy-to-use analyzer that is suitable for routine and emergency analyses in small laboratories.
科纳实验室20XT（芬兰热电电子公司）是一款用于比色法、免疫比浊法和离子选择性电极测量的临床化学分析仪。我们这项工作的目的是根据欧洲临床化学实验室标准指南评估科纳实验室20XT的分析性能。共检测了包括底物、酶、电解质和特定蛋白质在内的30种分析物。研究结果表明该分析仪精密度低（在所研究的所有三个水平下，大多数分析物的批内变异系数低于3.5%，日间变异系数小于2.5%）且准确性可接受。未发现明显的样品或试剂相关残留。结果表明该分析系统在规定的线性范围内运行。其结果与我们实验室常规使用的仪器（奥林巴斯AU2700、拜耳免疫比浊仪II）所获得的结果相比良好。总体而言，关于血红蛋白、高胆红素血症和浊度干扰的数据与已知事实相符。然而，发现轻微溶血会干扰碱性磷酸酶（ALP）检测，轻度脂血会影响葡萄糖检测。科纳实验室20XT是一款易于使用的分析仪，适用于小型实验室的常规和急诊分析。
