Gilger Brian C, Reeves Keri-Ann, Salmon Jacklyn H
College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
Vet Ophthalmol. 2005 Jul-Aug;8(4):265-9. doi: 10.1111/j.1463-5224.2005.00401.x.
To measure the ocular surface area, scleral thickness, and the aqueous and vitreous humor volumes in the canine and equine eye. Animals studied Fourteen canine and 16 equine cadaver eyes.
Eyes were measured either fresh or following fixation in formalin. Ocular surface area was calculated using the fluid displacement method. Globes were hemisected and aqueous and vitreous humor were collected and quantitated. Scleral thickness was measured by digital caliper, by image projection, and by ultrasonic biomicroscopy (UBM).
Mean +/- standard deviation (SD) scleral surface areas in canine and equine eyes were 12.87 +/- 2.24 and 57.23 +/- 5.63 cm2, respectively. Mean +/- SD aqueous humor volume was 0.77 +/- 0.24 in the dog and 3.04 +/- 1.27 mL in the horse. Mean vitreous humor volume was 1.7 +/- 0.86 mL for the canine eye and 26.15 +/- 4.87 mL for the equine eye. In canine and equine eyes, the sclera was thinnest at the ocular equator: 0.34 +/- 0.13 mm (canine) and 0.53 +/- 0.1 (equine). There were no significant differences between the direct caliper and projected thickness measurements or differences between measurements in the superior, inferior, nasal, and temporal quadrants of the eye. Scleral thickness in fresh tissue was greater than in fixed tissue at most sites.
The UBM measurement method appeared to be most consistent and reproducible when compared to direct measurement techniques. Formalin fixation of the eyes was associated with significantly thinner scleral tissue than with fresh ocular tissue.
测量犬和马眼的眼表面积、巩膜厚度以及房水和玻璃体体积。研究动物 14 只犬和 16 匹马的尸体眼。
眼睛在新鲜状态下或用福尔马林固定后进行测量。眼表面积采用液体置换法计算。将眼球切成两半，收集并定量房水和玻璃体。巩膜厚度通过数字卡尺、图像投影和超声生物显微镜（UBM）测量。
犬和马眼巩膜表面积的均值±标准差（SD）分别为 12.87±2.24 和 57.23±5.63 cm²。犬眼房水体积均值±SD 为 0.77±0.24 mL，马眼为 3.04±1.27 mL。犬眼玻璃体体积均值为 1.7±0.86 mL，马眼为 26.15±4.87 mL。在犬和马眼中，巩膜在眼赤道处最薄：犬为 0.34±0.13 mm，马为 0.53±0.1 mm。直接用卡尺测量和投影测量的厚度之间无显著差异，眼的上、下、鼻、颞象限测量值之间也无差异。大多数部位新鲜组织的巩膜厚度大于固定组织。
与直接测量技术相比，UBM 测量方法似乎最具一致性和可重复性。眼睛用福尔马林固定后，巩膜组织比新鲜眼组织显著更薄。
