Willemer S, Elsässer H P, Adler G
Department of Internal Medicine, Philipps University, Marburg, FRG.
Eur Surg Res. 1992;24 Suppl 1:29-39. doi: 10.1159/000129237.
Intravenous infusion of the synthetic cholecystokinin analogue cerulein at a dose of 0.25 micrograms/kg/h causes maximal stimulation of pancreatic exocrine secretion. The infusion of supramaximal doses of cerulein (5 and 10 micrograms/kg/h) induces a significant increase in pancreatic enzymes in blood, and interstitial edema and inflammatory cell infiltration. This model of hormone-induced pancreatitis works in rats, mice, dogs and hamsters. Besides intravenous infusion, repeated intraperitoneal injections can also be used for induction of pancreatitis. In the early phase of cerulein-induced pancreatitis, large autophagic vacuoles result from fusion of zymogen granules within the acinar cell. This is accompanied by an increase in lysosomal enzyme activity and activation of trypsinogen which finally leads to cellular necrosis. All animals survive the induction of pancreatitis. The pancreas completely regenerates within 6 days after induction of pancreatitis. This model of experimental pancreatitis favors the analysis of intracellular events in the early phase of pancreatitis.
以0.25微克/千克/小时的剂量静脉输注合成胆囊收缩素类似物雨蛙肽可引起胰腺外分泌的最大刺激。输注超最大剂量的雨蛙肽（5和10微克/千克/小时）会导致血液中胰腺酶显著增加，以及间质水肿和炎性细胞浸润。这种激素诱导性胰腺炎模型在大鼠、小鼠、狗和仓鼠中均有效。除静脉输注外，重复腹腔注射也可用于诱导胰腺炎。在雨蛙肽诱导的胰腺炎早期，腺泡细胞内的酶原颗粒融合形成大的自噬泡。这伴随着溶酶体酶活性的增加和胰蛋白酶原的激活，最终导致细胞坏死。所有动物在胰腺炎诱导后均存活。胰腺炎诱导后6天内胰腺完全再生。这种实验性胰腺炎模型有利于分析胰腺炎早期的细胞内事件。
