Chi J X, Huang L, Nie W, Wang J, Su B, Yang F
Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan 650223, People's Republic of China.
Chromosoma. 2005 Aug;114(3):167-72. doi: 10.1007/s00412-005-0004-x. Epub 2005 Jul 12.
The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of conserved chromosomal segments and the molecular mechanisms underlying the tandem fusions, we have constructed a highly redundant (more than six times of whole genome coverage) bacterial artificial chromosome (BAC) library of Indian muntjac. The BAC library contains 124,800 clones with no chromosome bias and has an average insert DNA size of 120 kb. A total of 223 clones have been mapped by fluorescent in situ hybridization onto the chromosomes of both Indian muntjac and Chinese muntjac and a high-resolution comparative map has been established. Our mapping results demonstrate that all tandem fusions that occurred during the evolution of Indian muntjac karyotype from the acrocentric 2n=70 hypothetical ancestral karyotype are centromere-telomere (head-tail) fusions.
印度麂（Muntiacus muntjak vaginalis）雌性的核型为2n = 6，雄性为2n = 7，近期的分子细胞遗传学研究已充分证明其核型通过广泛的串联融合和多次着丝粒融合而发生的进化过程。为了确定保守染色体片段的融合方向以及串联融合背后的分子机制，我们构建了一个高度冗余（超过全基因组覆盖度的六倍）的印度麂细菌人工染色体（BAC）文库。该BAC文库包含124,800个克隆，无染色体偏向性，平均插入DNA大小为120 kb。通过荧光原位杂交，共223个克隆已定位到印度麂和中国麂的染色体上，并建立了高分辨率的比较图谱。我们的定位结果表明，在印度麂核型从端着丝粒2n = 70假设祖先核型进化过程中发生的所有串联融合都是着丝粒 - 端粒（头 - 尾）融合。
