Rajah S V, Parslow J M, Howell R J, Hendry W F
Williamson Laboratory, St. Bartholomew's Hospital, London, United Kingdom.
Fertil Steril. 1992 Jun;57(6):1300-3. doi: 10.1016/s0015-0282(16)55091-2.
To compare two methods of detection of surface bound antibodies on spermatozoa from subfertile males.
Prospective comparison of direct mixed antiglobulin reaction (MAR) for immunoglobulin (Ig)G with direct immunobead test (IgG and IgA) applied to spermatozoa from male partners of infertile couples. Circulating unbound antibody measured by tray agglutination test in serum and seminal plasma in a representative proportion.
Seminology laboratory.
One hundred nine male partners of infertile couples.
Highly significant correlation between direct MAR (IgG) and direct immunobed test (IgG) and between both of these tests and serum unbound antibody measured by tray agglutination test. Highly significant correlation between direct immunobead test (IgA) and seminal plasma unbound antibody measured by tray agglutination test, but no correlation with MAR (IgG).
Mixed antiglobulin reaction (IgG) is a cheap, quick, and sensitive screening test, but immunobead test (IgA) provides useful additional information on class of antibody on spermatozoa that may be clinically more important.
比较两种检测男性不育患者精子表面结合抗体的方法。
对应用于不育夫妇男性伴侣精子的免疫球蛋白（Ig）G直接混合抗球蛋白反应（MAR）与直接免疫珠试验（IgG和IgA）进行前瞻性比较。通过托盘凝集试验对血清和精浆中循环游离抗体进行代表性比例的检测。
精液学实验室。
109对不育夫妇的男性伴侣。
直接MAR（IgG）与直接免疫珠试验（IgG）之间，以及这两种试验与通过托盘凝集试验检测的血清游离抗体之间存在高度显著相关性。直接免疫珠试验（IgA）与通过托盘凝集试验检测的精浆游离抗体之间存在高度显著相关性，但与MAR（IgG）无相关性。
混合抗球蛋白反应（IgG）是一种廉价、快速且灵敏的筛查试验，但免疫珠试验（IgA）可提供有关精子抗体类别方面有用的额外信息，这在临床上可能更为重要。
