Chen Hua, Li Ping, Liu Jing, Li Yin-Xin
Key Laboratory of Phytosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093.
Sheng Wu Gong Cheng Xue Bao. 2005 Mar;21(2):244-9.
A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific bands.
本文介绍了一种从叶片和叶柄外植体直接和间接再生普通蒲公英（Taraxacum officinale Weber）的方案。在含有0.2 mg/L吲哚乙酸（IAA）和1 mg/L噻苯隆（TDZ）的MS培养基上获得了多个芽。对于间接再生，在添加了0.5 mg/L 2,4-二氯苯氧乙酸（2,4-D）和2.0 mg/L 6-苄基腺嘌呤（6-BA）的MS培养基上，从叶片和叶柄外植体获得了易碎愈伤组织。当这些愈伤组织在含有1.0 mg/L 6-BA的MS培养基上培养时，获得了再生植株。对9株再生植株进行的随机扩增多态性DNA（RAPD）分析显示，从10个引物中得到了61条可计分条带，其中包括3条特异性条带。
