Li Ping, Chen Hua, Li Yin-Xin
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093.
Sheng Wu Gong Cheng Xue Bao. 2005 Nov;21(6):916-22.
Plantago major is not only used as medicinal herb but also an important model plant of ecology. Little work has been reported on the tissue culture of P. major. A reproducible system for direct shoot morphogenesis and callus induction of Plantago major L. 'Giant Turkish' was described. Using seed as explants, the adventitious buds were obtained 4 to 5 weeks following incubation on MS medium supplemented with 0.2 mg/L IAA and 1.0 mg/L TDZ. The frequency of adventitious buds was as high as 100%. The average number of buds per explant was 14.6. Random amplified polymorphic DNA analysis on 9 regenerants indicated that somaclonal variation occurred at DNA level. Using leaves as explants, calli were easily induced on MS medium supplemented with 1.0 mg/L NAA 3 weeks following inoculation. The frequency of callus induction can be as high as 98%. On MS medium containing 4.0 mg/L 6-BA, 25% of calli differentiated and the mean number of buds per piece of callus was 2.8. The buds developed roots on 11/2 MS medium and formed plantlets, 90% of which survived when transplanted to greenhouse.
大车前不仅被用作草药，也是一种重要的生态模式植物。关于大车前组织培养的报道较少。本文描述了一种可重复的大车前‘巨型土耳其’直接芽形态发生和愈伤组织诱导体系。以种子为外植体，在添加0.2 mg/L IAA和1.0 mg/L TDZ的MS培养基上培养4至5周后获得不定芽。不定芽发生率高达100%。每个外植体平均芽数为14.6。对9个再生植株进行随机扩增多态性DNA分析表明，在DNA水平上发生了体细胞克隆变异。以叶片为外植体，接种3周后在添加1.0 mg/L NAA的MS培养基上很容易诱导出愈伤组织。愈伤组织诱导率可高达98%。在含有4.0 mg/L 6 - BA的MS培养基上，25%的愈伤组织分化，每块愈伤组织平均芽数为2.8。芽在1/2MS培养基上生根并形成植株，其中90%移栽到温室后存活。
