从腹泻患儿中分离出的河流弧菌的细胞毒性和细胞空泡化活性。

Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea.

作者信息

Chakraborty Rupa, Chakraborty Subhra, De Keya, Sinha Sutapa, Mukhopadhyay Asish K, Khanam Jasmina, Ramamurthy Thandavarayan, Takeda Yoshifumi, Bhattacharya Sujit K, Nair G Balakrish

机构信息

National Institute of Cholera and Enteric Diseases, P-33, CIT Road, Beliaghata, Kolkata - 700 010, India 2Jadavpur University, Jadavpur, Kolkata - 700 032, India 3Jissen Women's University, 4-1-1, Osakane Hinocity, Tokyo 191-8510, Japan 4International Centre for Diarrhoeal Diseases Research, Bangladesh, Mohakhali, Dhaka - 1212, Bangladesh.

出版信息

J Med Microbiol. 2005 Aug;54(Pt 8):707-716. doi: 10.1099/jmm.0.45820-0.

DOI:10.1099/jmm.0.45820-0

PMID:16014422

Abstract

Vibrio fluvialis is a halophilic Vibrio species associated with acute diarrhoeal illness in humans. It has the potential to cause outbreaks and has an association with paediatric diarrhoea. In this study, 11 V. fluvialis strains isolated from hospitalized patients with acute diarrhoea at the Infectious Diseases Hospital, Kolkata were extensively characterized. All the strains showed growth in peptone broth containing 7% NaCl. The strains showed variable results in Voges-Proskauer test and to a vibriostatic agent. There was also variation in their antibiograms, and some of the strains were multidrug resistant. Among the 11 strains, two showed only a single band difference in their PFGE profile and the remaining strains showed nine different PFGE patterns. However, unlike PFGE, the strains exhibited close matches and clustering in their ribotype patterns. The haemolytic effect on sheep red blood cells varied with strains. Partial sequence analysis revealed that the V. fluvialis haemolysin gene has 81% homology with that of the El Tor haemolysin of Vibrio cholerae. A striking finding was the capability of all the strains to evoke distinct cytotoxic and vacuolation effects on HeLa cells.

摘要

河流弧菌是一种嗜盐弧菌，与人类急性腹泻疾病有关。它有可能引发疫情，且与小儿腹泻有关。在本研究中，对从加尔各答传染病医院的急性腹泻住院患者中分离出的11株河流弧菌菌株进行了全面表征。所有菌株在含7%氯化钠的蛋白胨肉汤中均能生长。这些菌株在Voges-Proskauer试验和对一种弧菌抑制剂的反应中显示出不同结果。它们的抗菌谱也存在差异，一些菌株具有多重耐药性。在这11株菌株中，有两株在脉冲场凝胶电泳（PFGE）图谱上仅显示一条带的差异，其余菌株显示出九种不同的PFGE模式。然而，与PFGE不同的是，这些菌株在核糖体分型模式上表现出紧密匹配和聚类。对绵羊红细胞的溶血作用因菌株而异。部分序列分析表明，河流弧菌溶血素基因与霍乱弧菌埃尔托溶血素基因有81%的同源性。一个显著的发现是，所有菌株都能够对HeLa细胞产生明显的细胞毒性和空泡化作用。