Schmidt-Ott Ruprecht, Brass Felicitas, Scholz Christiane, Werner Carola, Groß Uwe
Institute of Medical Microbiology1 and Department for Medical Statistics2, University of Göttingen, D-37075 Göttingen, Germany.
J Med Microbiol. 2005 Aug;54(Pt 8):761-767. doi: 10.1099/jmm.0.46040-0.
Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient groups. In order to obtain reliable epidemiological data as to the role of C. jejuni in causing late-onset complications, a highly specific and sensitive diagnostic tool for the epidemiological investigation of C. jejuni-associated diseases was developed. It was shown that recombinant proteins encoded by the C. jejuni genes cj0017 (P39) and cj0113 (P18) are specifically recognized by antibodies in sera from patients with C. jejuni enteritis. An ELISA using recombinant P18 and P39 as antigens was 91.9% sensitive and 99.0% specific, with positive and negative predictive values of 97.1% and 97.0%, respectively, comparing favourably with the 27.0% sensitivity of a routinely used serological assay.
空肠弯曲菌是感染性腹泻的常见病因，并且越来越被认为是迟发性并发症的诱因。常用血清学检测的标准化程度低，这可能解释了在特定患者群体中关于既往空肠弯曲菌感染频率的相互矛盾的结果。为了获得关于空肠弯曲菌在引起迟发性并发症中作用的可靠流行病学数据，开发了一种用于空肠弯曲菌相关疾病流行病学调查的高度特异性和敏感性的诊断工具。结果表明，空肠弯曲菌基因cj0017（P39）和cj0113（P18）编码的重组蛋白被空肠弯曲菌肠炎患者血清中的抗体特异性识别。以重组P18和P39作为抗原的ELISA敏感性为91.9%，特异性为99.0%，阳性和阴性预测值分别为97.1%和97.0%，与常规使用的血清学检测27.0%的敏感性相比更具优势。
