Maeland J A, Bevanger L, Enge J
Department of Microbiology, Faculty of Medicine, University of Trondheim, Norway.
APMIS. 1993 Aug;101(8):647-50. doi: 10.1111/j.1699-0463.1993.tb00159.x.
Single serum specimens forwarded for Salmonella and Yersinia serology, from a total of 250 patients, were tested for anti-C. jejuni antibodies of the IgG class. Anti-Salmonella and anti-Y. enterocolitica O3 antibodies were examined by a microagglutination test and anti-C. jejuni antibodies by ELISA against a C. jejuni ultrasonicate before (ELISA) and after neutralization of antibodies which cross-reacted with Helicobacter pylori antigens (ELISA-Abs). Blood donor sera (n = 50) and sera (n = 40) from patients with various infectious diseases served as controls. A positive test for anti-Salmonella antibodies was recorded in 4/250 (1.6%) of the patients, for anti-Yersinia antibodies in 7/250 (2.8%), and for anti-C. jejuni antibodies in 7/250 (2.8%) in the ELISA; in 25/250 (10%) in the ELISA-Abs. No mixed infection was recorded by the serological testing. The ELISA-Abs showed a diagnostic specificity of 97.7%. Our results support the inference that diagnostic serology for enteropathogenic bacteria should include serology for C. jejuni, preferably by tests which exclude participation by antibodies which cross-react with H. pylori antigens.
从总共250名患者中送检用于沙门氏菌和耶尔森氏菌血清学检测的单份血清标本，检测了IgG类抗空肠弯曲菌抗体。通过微量凝集试验检测抗沙门氏菌和抗小肠结肠炎耶尔森氏菌O3抗体，通过ELISA检测针对空肠弯曲菌超声破碎物的抗空肠弯曲菌抗体，检测前（ELISA）和中和与幽门螺杆菌抗原交叉反应的抗体后（ELISA-Abs）。献血者血清（n = 50）和患有各种传染病患者的血清（n = 40）用作对照。ELISA检测中，250名患者中有4名（1.6%）抗沙门氏菌抗体检测呈阳性，7名（2.8%）抗耶尔森氏菌抗体检测呈阳性，7名（2.8%）抗空肠弯曲菌抗体检测呈阳性；ELISA-Abs检测中，250名患者中有25名（10%）呈阳性。血清学检测未记录到混合感染。ELISA-Abs显示诊断特异性为97.7%。我们的结果支持这样的推断，即肠道致病菌的诊断血清学应包括空肠弯曲菌血清学检测，最好通过排除与幽门螺杆菌抗原交叉反应抗体参与的检测方法。
