Herbrink P, van den Munckhof H A, Bumkens M, Lindeman J, van Dijk W C
Department of Pathology, Stichting Samenwerking Delftse Ziekenhuizen, Delft, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1988 Jun;7(3):388-93. doi: 10.1007/BF01962343.
An ELISA for detection of IgG, IgA, and IgM antibody using an acid-glycine extract from Campylobacter jejuni as antigen was developed. To determine the value of this assay for the diagnosis of acute Campylobacter jejuni infections, the IgG, IgA, and IgM immune response against Campylobacter jejuni was investigated at various timepoints after infection in patients with culture-proven infection. A total of 112 sera from 46 patients and 78 sera from a control group were tested. All but one of the 46 patients with culture-proven Campylobacter jejuni enteritis developed IgG antibodies against Campylobacter jejuni. IgA and IgM ELISA both showed 97% specificity, and sensitivity of 63% and 30% respectively. IgG antibody titers generally remained at a constant level for more than 50 days, whereas IgA and IgM antibody titers declined more rapidly to normal values within 30 to 50 days after onset of clinical symptoms. Detection of Campylobacter jejuni specific IgA antibodies in a single serum sample provided the most useful assay for serological diagnosis of Campylobacter jejuni enteritis. The presence of Campylobacter jejuni specific IgM antibodies was the sole diagnostic criterion in three cases. Serological diagnosis of Campylobacter jejuni enteritis should therefore include both IgA and IgM antibody determination.
开发了一种酶联免疫吸附测定法（ELISA），该方法使用空肠弯曲菌的酸 - 甘氨酸提取物作为抗原，用于检测IgG、IgA和IgM抗体。为了确定该检测方法在诊断急性空肠弯曲菌感染中的价值，对经培养证实感染的患者在感染后的不同时间点，研究了针对空肠弯曲菌的IgG、IgA和IgM免疫反应。共检测了46例患者的112份血清以及对照组的78份血清。46例经培养证实为空肠弯曲菌肠炎的患者中，除1例之外，其余患者均产生了针对空肠弯曲菌的IgG抗体。IgA和IgM ELISA的特异性均为97%，敏感性分别为63%和30%。IgG抗体滴度通常在50多天内保持恒定水平，而IgA和IgM抗体滴度在临床症状出现后30至50天内更快地下降至正常值。在单个血清样本中检测空肠弯曲菌特异性IgA抗体，为空肠弯曲菌肠炎的血清学诊断提供了最有用的检测方法。在3例病例中，空肠弯曲菌特异性IgM抗体的存在是唯一的诊断标准。因此，空肠弯曲菌肠炎的血清学诊断应包括IgA和IgM抗体的测定。
